摘要
A simple and sensitive method for the separation and determination of isoproterenol from other doping drugs has been developed on thin layers of bismuth silicate,a synthetic inorganic ion exchanger as adsorbent in thin layer chromatography(TLC).A mixture of methanol and 0.1 mol/L formic acid(3∶7,v/v)was employed as the mobile phase.The development time was 32 min.The quantitative measurement were performed with a Camag TLC Scanner-3 at wavelength(λ)of 410 nm.The isoproterenol recovery in this procedure was 98.9%.The linear correlation coefficient was greater than 0.987 1 and the relative standard deviation(RSD)was less than 0.94.The limit of detection(LOD)and limit of quantification(LOQ)were 7.7×10-7mol/L and 3.85×10-6mol/L,respectively.This method has been applied in the determination of isoproterenol in dosage forms and in biological fluids.
A simple and sensitive method for the separation and determination of isoproterenol from other doping drugs has been developed on thin layers of bismuth silicate,a synthetic inorganic ion exchanger as adsorbent in thin layer chromatography（TLC）.A mixture of methanol and 0.1 mol/L formic acid（3∶7,v/v）was employed as the mobile phase.The development time was 32 min.The quantitative measurement were performed with a Camag TLC Scanner-3 at wavelength（λ）of 410 nm.The isoproterenol recovery in this procedure was 98.9%.The linear correlation coefficient was greater than 0.987 1 and the relative standard deviation（RSD）was less than 0.94.The limit of detection（LOD）and limit of quantification（LOQ）were 7.7×10^-7mol/L and 3.85×10^-6mol/L,respectively.This method has been applied in the determination of isoproterenol in dosage forms and in biological fluids.
出处
《色谱》
CAS
CSCD
北大核心
2015年第6期667-671,共5页
Chinese Journal of Chromatography
基金
Financial assistance was provided by Kharazmi University,Research Council
