预处理方法对海洋微藻脂肪酸萃取量及其碳稳定同位素组成的影响

Effects of pretreatment methods on the extraction amount and the stable carbon isotopic composition of marine microalgae fatty acid

为探讨不同前处理对海洋微藻脂肪酸萃取量及碳稳定同位素比值(δ13C)的影响,本文应用GC/MS和GC/IRMS方法,测得不同酸催化条件下产物(脂肪酸甲酯)的相对含量及单分子脂肪酸甲酯δ13C值,并分析比较。结果表明:对于新鲜微藻样品,盐酸催化比硫酸催化更适合于微藻中多不饱和脂肪酸的甲酯化;用硫酸催化时,微藻样品在不同预处理(A-新鲜微藻样品、B-干燥处理、C-溶剂提取)下,测得的微藻脂肪酸甲酯相对含量及δ13C值也存在一定差异。

To explore the effects of different pretreatments on the extraction amount and carbon stable isotope ratio （δ13C ） of fatty acid in Marine microalgae ,the relative content and δ13C values of the products of fatty acid methyl esters （FAMEs） were measured by GC/MS and GC/IRMS under the different acid catalytic condition,and then were com- pared and analyzed. The results show that hydrochloric acid catalysis is more suitable for fresh samples of microalgae than sulfuric acid in the process of producing polyunsaturated FAMEs ; When sulfuric acid was used as catalysis, the relative contents and δ13C values of FAMEs also exist certain differences in different pretreatments （ A-fresh samples of microalgae, B-dry processing, C-solvent extraction） of the microalgae samples.