类叶牡丹化学成分的研究

Saponin constituents from roots and rhizomes of Caulophyllum robustum

目的对类叶牡丹Caulophyllum robustum根中化学成分进行研究。方法利用反复硅胶柱色谱、中压柱色谱及半制备液相色谱等方法分离纯化，通过核磁共振谱、质谱等光谱数据鉴定化合物结构。结果从类叶牡丹根中分离得到10个化合物，分别鉴定为刺囊酸-3-Oβ-D-葡萄吡喃糖-（1→2）-α-L-吡喃阿拉伯糖苷（1）、3-O-α-L-吡喃阿拉伯糖-常春藤皂苷元-28-O-β-D-葡萄吡喃糖-（1→6）-β-D-吡喃葡萄糖苷（2）、HN-saponinH（3）、ciwujianosides A1（4）、glycoside L-K1（5）、3-O-β-D-吡喃葡萄糖-（1→3）-α-L-吡喃阿拉伯糖-常春藤皂苷元-28-O-α-L-吡喃鼠李糖-（1→4）-β-D-吡喃葡萄糖-（1→6）-β-D-吡喃葡萄糖苷（6）、leonticin F（7）、3-O-β-D-吡喃葡萄糖-（1→3）[β-D-吡喃葡萄糖-（1→2）]-α-L-吡喃阿拉伯糖-刺囊酸-28-O-α-L-吡喃鼠李糖-（1→4）-β-D-吡喃葡萄糖-（1→6）-β-D-吡喃葡萄糖苷（8）、leonticin A（9）、莫诺苷（10）。结论化合物10是环烯醚萜类，其余化合物为皂苷类；化合物1、10首次从红毛七属植物中分离得到，化合物2～9首次从该植物中分离得到。

Objective To separate the saponins from the roots and rhizomes of Caulophyllum robustum and determine their chemical structures. Methods The chemical constituents were isolated by repeated silica gel chromatography, medium pressure columψ chromatography, and semi-preparative liquid chromatography. Their structures were elucidated by the data of NMR and MS. Results Ten compounds were isolated from the roots and rhizomes of C, robustum and the structures of compounds 1-10 were identified as echinocystic acid-3-O-β-D-glucopyranosyl-（1→2）-α-L-arabinopyranoside （1）, 3-O-α-L-arabinopyranosylhederagenin-28-O-β-D- glucopyranosyl-（1→6）-β-D-glucopyranoside （2）, HN-saponin H （3）, ciwujianosides A1 （4）, glycoside L-K1 （5）, 3-O-β-D- glucopyranosyl-（1→3）-α-L-arabinopyranosyl-hederagenin-28-O-α-L-rhamnopyranosyl-（1-→4）-β-D-glucopyranosyl-（1→6）-β-D-glucopyranoside （6）, leonticin F （7）, 3-O-β-D-glucopyranosyl-（1→3） [β-D-glucopyranosyl-（1→2）] α-L-arabinopyranosyl-echinocystic acid-28-O-α-L-rhamnopyranosyl-（→4）-β-D-glucopyranosyl-（1→6）-β-D-glucopyranoside （8）, leonticin A （9）, and morroniside （10）. Conclusion Compound 10 is a iridoid. Compounds 1 and 10 are firstly isolated from the plants of Cautoptiyllum Maxim., compouds 2-9 are isolated from this plant for the first time.