无绿藻分子生物学鉴定与分型研究新进展

New advance in the study of the molecular biological identification and typing of Prototheca

以基因序列为鉴定基础的分子生物学方法以其客观、可重复性好等优点,被认为是分子鉴定无绿藻的"金标准"。该文对近年来应用在无绿藻鉴定和分型方面的实时定量PCR(Real-Time Quantitative PCR,RT-PCR)技术、单链构象多态性(Single Stranded Conformation Polymophism,SSCP)分析、限制性片段长度多态性(Restriction Fragment Length Polymorphism,RFLP)分析、高分辨率熔解曲线(High Resolution Melting,HRM)分析、质谱(Mass Spectrometry,MS)分析等分子生物学新技术和新方法进行综述。

The molecular biology method based on gene sequencing which is objective,highly reproducible and so on,is considered the “gold standard” of Prototheca ＇s molecular identification.To summarize the new molecular biology methods and techniques applied to Prototheca＇s identification and typing,such as realtime quantitative PCR （RTPCR） technology,single strand conformation polymorphism （ SSCP ） analysis, PCRrestriction fragment length polymorphism （ RFLP ） analysis, high resolution melting （HRM） analysis,mass spectrometry（MS） analysis.