基于蛋白质组学的药物代谢酶与转运体定量分析研究进展

Recent advances in the quantification of drug metabolizing enzymes and transporters by proteomic approach

随着药物开发过程的不断完善和研究技术的进步,人们对药物在体内的代谢过程和代谢机制有了更深层次的认识。药物在体内的代谢由药物代谢酶和转运体共同介导,因此,对药物代谢酶和转运体的研究已经成为药物研发过程中的重要内容。传统免疫测定法不能真实反映体内药物代谢酶和转运体的表达水平,且存在灵敏度低、特异性差的缺点。本文主要从蛋白质组学的角度出发,结合近年来生物质谱技术的发展,对药物代谢酶和转运体的定量研究做一综述介绍。

With the advance of drug development and research techniques, the drug metabolic processes and mechanism can be more deeply achieved. As the drug metabolism and pharmacokinetics process are mediated by drug metabolizing enzymes and transporters, study of drug metabolizing enzymes and transporters has become an important part for drug development. The traditional immunoassays with low sensitivity and poor specificity can not reflect the accurate expression level of drug metabolizing enzymes and transporters. We now give a brief review on the quantitative study of drug metabolizing enzymes and transporters by mass spectrometry-based proteomic approach.