组培增殖方式对网纹草嵌合性状稳定性的影响

Studies of the Chimeric Stability of Fittonia albivenis with Different Proliferation Methods of Tissue Culture

以一种具有良好的谱系细胞标记的周缘嵌合体网纹草(Fittonia albivenis)为研究材料,对不同增殖方式下其组培苗嵌合性状的稳定性进行了观察分析。结果表明,以茎段诱导腋芽增殖时网纹草的稳定性很高,但以丛生芽增殖时其嵌合性状发生了变异,变异率为21.32%;叶片组织解剖学观察结果显示,母本嵌合体茎尖分生组织中红和绿2种细胞谱系的细胞层排布在丛生芽再生过程中发生了改变,这可能是导致新类型嵌合体产生的原因。研究结果不仅对植物嵌合体种苗的组培生产具有重要的指导意义,而且为嵌合体的种质创新工作提供了新方法。

The chimeric stability of Fittonia albivenis with different proliferation methods was analysed, with assistance from favourable markers of cell lineage. The axillary bud proliferation was appropriate for the multiplication of ornamental chimera plants; the variation rate of cluster-bud proliferation was 21.32%, and the two lineages with red and green color separately were found rearranged in the shoot apical meristem of cluster buds, which might directly induce the appear- ance of new chimera types. This study offers insights into the production of chimera plantlets via tissue culture and a new method for producing innovative germplasm for plant chimeras.