克拉霉素对小鼠肺炎支原体感染模型IL-10表达的影响

Effect of Clarithromycin on IL-10 expression in lung of mice with mycoplasma pneumoniae infection

目的通过建立肺炎支原体(MP)感染BALB/c小鼠的模型,探讨克拉霉素对肺炎支原体感染小鼠IL-10表达的影响。方法 60只昆明小鼠随机分为对照组、MP感染组及克拉霉素治疗组,每组20只,建立小鼠肺炎支原体感染模型,克拉霉素治疗组用克拉霉素治疗5 d。HE染色观察病理组织学变化;Western blot方法检测各组小鼠肺组织IL-10的表达;RT-PCR方法检测各组小鼠肺组织IL-10m RNA的表达水平。结果 MP感染后,小鼠肺组织呈间质性炎性改变,肺泡间隔增宽,大量淋巴细胞和巨噬细胞浸润在支气管、血管周围,肺泡内有渗出。相比于对照组,MP感染组小鼠肺组织IL-10蛋白和m RNA表达水平明显降低(<0.01);与MP感染组相比,克拉霉素治疗组小鼠肺组织IL-10蛋白和m RNA表达水平明显升高(<0.01)。结论克拉霉素可显著上调肺炎支原体感染小鼠肺组织IL-10水平。

Objective To study the effect of Clarithromycin on the expression of interleukin 10（IL-10） in lung of mice with mycoplasma pneumoniae infection. Methods 60 Kunming mice were randomly averagely divided into control group, MP infection group and clarithromycin treatment group. Hematoxylin and eosin（HE） staining was performed for the observation of histological changes, the expression of IL-10 protein and mRNA in lung tissues was detected by Western blot and RT-PCR respectively. Results Interstitial inflammatory changes were found after MP infection, such as widened alveolar septum, the infiltration of lymphocytes and macrophages in bronchial and perivascular, alveolar exudation. Compared with control group, the expression level of IL-10 protein and mRNA was significantly lower in mice of MP infection group（P〈0.01）, but significantly higher in clarithromycin treatment group than in MP infection group（P〈0.01）. Conclusion Clarithromycin upregnlates the level of IL-10 protein and mRNA in lung of mice with mycoplasma pneumoniae infection.