摘要
目的组蛋白去乙酰化酶抑制剂(Trichostatin A,TSA)处理人THP-1细胞,干预组蛋白H3乙酰化水平,探讨组蛋白H3乙酰化对人THP-1细胞中TLR2基因表达水平的影响。方法构建THP-1巨噬细胞模型,分别利用不同浓度TSA处理细胞,Real-time PCR和Western blot方法检测m RNA和蛋白的表达;染色质免疫共沉淀技术(Chromatin immunoprecipitation,Ch IP)比较TSA处理前后启动子区H3乙酰化水平。结果TSA以浓度依赖方式上调表达水平。TSA上调组蛋白H3乙酰化水平,使启动子区H3乙酰化水平明显上升。结论 TSA通过组蛋白H3乙酰化影响基因表达,这是乙酰化调控TLR2基因表达的一种可能机制。
Objective To determine the effect of histone acetylation H3 on the expression of TLR2 gene in human differentiated THP-1 cells by histone deaeetylase inhibitor, trichostatin A （TSA）. Methods The THP-1 macrophage model was constructed, TLR2 expression was detected by real-time PCR and Western blot with different concentrations of TSA. Chromatin immunoprecipitation（ChIP） was used to analyze the acetylation of histone H3 in the promoter regions of TZR2 . Results TSAtreatmentresultedinobviouslyincreaseoftheexpressionlevelof TZR2 in a dose dependent manner, and upregulated the aeetylation level of histones H3 in the promoter region of TZR2 . Conclusion TSA upregulates TZR2 gene expression by histone H3 acetylation.
出处
《解剖科学进展》
CAS
2015年第3期313-315,共3页
Progress of Anatomical Sciences
基金
国家自然科学基金资助项目(No.81201333)
