中国职业暴露人群感染H9N2禽流感病毒血清学调查

Serological study of human infection with avian influenza A(H9N2) virus in population with occupational exposure in China

目的评估我国职业暴露人群感染H9N2禽流感病毒的风险因素。方法利用2009 2011年间在我国22个省(直辖市、自治区)采集的职业暴露人群血清13 715份,筛选了在我国主要流行的G9系毒株A/Chicken/AK4/Anhui/2011作为检测抗原,使用血凝抑制方法初筛和微量中和方法复核两种血清抗体检测方法,在我国大陆地区开展系统的H9N2禽流感病毒血清学研究。结果 H9N2禽流感病毒在中国职业暴露人群的中和抗体血清阳性率为0.41%(63份阳性血清标本)。所有家禽职业暴露人群均有阳性血清标本检出,活禽市场暴露人群感染H9N2禽流感病毒风险显著高于其他职业暴露人群。结论活禽市场是人感染禽流感病毒的重要暴露风险因素。加强H9N2禽流感病毒在我国职业暴露人群的监测,对于有效评估和防控禽流感对人类的危害具有重要的意义。

Objective To evaluate the risk of human infection with avian influenza A（H9N2） virus in population with occupational exposure in China.Methods A total of 13,715 serum samples were collected from people with occupational exposures in 22 provinces of China.Hemagglutination inhibition（HI） test was conducted to detect these serum samples and micro neutralization（MN） assay was used to confirm the results by using G9 lineage virus A /Chicken / AK4 / Anhui /2011 as antigen.Results Among the serum samples detected,63 were H9N2 virus positive（0.41%）.Positive serum samples were detected in all the people with occupational exposure to poultry.The seroprevalence in workers in live poultry market was significantly higher than that in other groups.Conclusion Exposure to live poultry was the major risk factor for human infection with avian influenza virus.It is necessary to strengthen the surveillance for H9N2 virus infection in population with occupational exposure in China,which will benefit the effective prevention and control of human infection with avian influenza virus.