CB6F1小鼠黑色素瘤B16-F10细胞移植瘤模型的建立

Establishment of tumor model with B16-F10 melanoma in CB6F1 mice

目的 在CB6F1小鼠上建立黑色素瘤B16-F10细胞皮下移植瘤和肺转移瘤模型,为黑色素瘤的单倍体相合淋巴细胞输注的实验研究提供新的动物模型.方法 将B16-F10细胞株于CB6F1小鼠皮下及尾静脉注射接种,将小鼠按随机数字表法分成3组,即高剂量组（2× 105细胞）、中剂量组（1×105细胞）、低剂量组（5×104细胞）,每组8只.筛选出最适接种细胞数后,分别建立皮下移植瘤和肺转移瘤模型,实验重复3次,观察成瘤率和荷瘤鼠的生存时间.结果 在皮下种植瘤模型中,高、中、低剂量组的小鼠成瘤分别为8、8、6只;在肺转移瘤模型中,高、中、低剂量组的小鼠肺转移瘤分别为8、8、5只.以1×105细胞接种建立的皮下移植瘤和肺转移瘤模型重复3次,每次5只小鼠均成瘤.结论 建立了一个成瘤率高、操作简单、稳定的小鼠黑色素瘤模型,为研究单倍体相合淋巴细胞输注提供理想的小鼠实体瘤模型.

Objective To established subcutaneous tumor and pulmonary metastasis models with B16-F10 melanoma in CB6F1 mice,and provide a new melanoma-bearing mice model for haploidentical lymphocytes infusion.Methods CB6F1 mice were inoculated subcutaneously and injected intravenously by tail vein with B16-F10 melanoma cells.According to different number of inoculated B16-F10 cells,CB6F1 mice were randomly divided into three groups （high dose group：2×105 cells; medium dose group：1×105 cells;low dose group：5×104 cells）,and chose the best fit number of inoculated cells for establishing subcutaneous transplanted and metastatic melanoma models.Experiments were repeated three times.The incidence of tumorgenesis and survival time of tumor-bearing mice were examined.Results In the subcutaneous tumor models,the incidence of tumorgenesis were 8,8 and 6 in high,medium and low dose group,respectively.In the pulmonary metastasis models,the incidence of tumorgenesis were 8,8 and 5 in high,medium and low dose group,respectively.Then 1×105 cells were injected into mice,and the incidence of tumorgenesis were all 8/8 in both groups.Conclusion The results show that ideal melanoma-bearing mice models for haploidentical lymphocytes infusion are successfully established.These models are convenient,repeatable,and have high rate of bearing tumor.