基于药物体系的肉桂特征图谱质量表征关联分析研究

Quality representation and correlation analysis of the characteristic spectrum of Rougui based on drug system

目的建立基于药物体系的肉桂特征图谱质量表征关联分析方法,全面评价肉桂饮片质量。方法采用HPLC-PDA法建立肉桂饮片特征图谱,筛选7个特征峰并通过相对保留时间表征其质的关联性;同时测定6个主要有效指标性成分(原儿茶酸、表儿茶素、香豆素、肉桂醇、肉桂酸、肉桂醛)的含量并表征主要酚类成分和主要苯丙素类成分的含量,通过关联度等概念表征其量的关联性。结果以批号13为基准,基于质的表征模式及关联分析,肉桂饮片特征图谱中筛选的7个特征峰中有酚类成分2个、苯丙素类成分5个,15批肉桂饮片色谱图中均含有此7个特征峰。基于量的表征模式及关联分析,批号14有效指标性成分总体含量高于基准批号13,批号4、6、8、10含量与基准批号13关联性较高,综合评价得出批号14、13、4、6、8、10优良度居前。结论在定性的基础上进行定量表征,可全面评价肉桂饮片质量优次,为肉桂饮片资源筛选、药物原料质量控制及应用提供依据,同时为中药质量评价提供新的方法。

Objective To establish quality representation and correlation analysis method for analyzing the characteristic spectrum of Rougui （ Cinnamomum cassia Presl.） based on drug system, evaluating the quality of Rougui decocting pieces comprehensively.Methods HPLC-PDA method was adopted to establish the characteristic spectrum of Rougui decocting pieces, and seven characteristic peaks were screened.The qualitative relevance was presented with the relative retention time characterization. Simultaneously, six main effective index components （ protocatechuic acid, epicatechin, coumarin, cinnamic alcohol, cinnamic acid, and cinnamaldehyde） were measured, and characterization contents of the main phenols and the main phenylpropanoids were presented, whose quantitative relevance was represented with correlation analysis.Results Using batch 13 as reference standards, based on the qualitative representation model and correlation analysis, seven characteristic peaks of Rougui decocting pieces were detected to have two characteristic peaks of phenols and five characteristic peaks of phenylpropanoids;the seven characteristic peaks were found in 15 batches of Rougui decocting pieces chromatograms .Based on the quantitative representation model and correlation analysis, the overall amount of effective index components in batch 14 were higher than those in batch 13; the levels of components in batch 4, 6, 8, and 10 were highly related to batch 13.Comprehensive evaluation result showed that the quality of batch 14, 13, 4, 6, 8, and 10 were comparatively higher.Conclusion Quality representation was carried out on the basis of the qualitative characterization.This method could evaluate Rougui pieces’ quality comprehensively, provide a basis for the screening of resources, for con-trolling the quality of pharmaceutical raw materials, and for the related application, and also provide a new methodology for quality evaluation of Chinese materia medica.