UPLC-MS/MS快速测定食物中毒样品中的酵米面黄杆菌毒素A

Rapid determination of Farinofermentans flavobacterium toxin A in foods from incident food poisoning by UPLC-MS / MS

目的建立超高效液相色谱-串联质谱（UPLC-MS/MS）法快速测定食物中毒样品中酵米面黄杆菌毒素A的方法。方法被椰毒假单胞菌酵米面亚种污染的食物样品经6 mol/L HCl酸化,用正己烷提取,经Waters HILIC C18（2.1 mm×100 mm,1.8μm）分离,在ESI-模式下用串联质谱仪定性定量分析。结果酵米面食物样品中酵米面黄杆菌毒素A的方法检出限为0.25μg/kg,2个水平的加标回收率为76.8%~101.6%,相对标准偏差均为8.5%。结论该方法测定酵米面食物样品中的酵米面黄杆菌毒素A快速、准确、可靠,灵敏度高。

Objective To establish a rapid method for determining Farinofermentans flavobacterium toxin A in samples of foods from incident food poisoning by high performance liquid chromatography - mass spectrometry/mass spectrometry （ UPLC - MS/ MS）. Methods Food samples contaminated by Pseudomonas cocovenenans subsp food samples were acidified by 6mol/L HCl, extracted by hexane, separated in waters HILIC C18 （2.1 mm × 100 mm, 1.8 μm） chromatographic column, and then quantita- tively analyzed by MRM tandem mass spectrometry qualitative and analyzer in the ESI- negative ion mode. Results The limits of detection （LOD） for farinofermentans flavobacterium toxin A was in the range of 0. 25 μg/kg. The recovery rate at the two spiked levels were 76.8% - 101.6%, and both the relative standard deviation （RSD） were less than 8.5%. Conclusion The rnethod can be used to determine farinofermentans flavobaeterium toxin A in samples of foods rapidly, accurately and sensitively.