ERCC1和TYMS的表达与晚期非小细胞肺癌患者采用替吉奥联合铂类化疗疗效的关系研究

Study on the relationship between ERCC1 and TYMS expression with the S-1 combined with platinum chemotherapy effect of non-small cell lung cancer patients

目的探讨ERCC1和TYMS的表达与晚期非小细胞肺癌患者采用替吉奥(S-1)联合铂类药物化疗近期疗效与远期生存的关系。方法随访68例接受姑息性肺癌切除治疗,同时采用替吉奥联合顺铂化疗的晚期(ⅢB/Ⅳ)非小细胞肺癌患者。采用实时荧光定量PCR法检测肿瘤组织ERCC1和TYMS的表达,经Pearsonχ2检验分析比较各基因表达与对应药物化疗疗效相关性,Cox回归分析筛选影响预后的独立危险因子。结果 ERCC1低表达组化疗有效率(CR+PR)明显高于高表达组(P<0.01);TYMS低表达组化疗有效率明显高于高表达组(P<0.01)。多因素Cox回归分析显示:PS评分、临床分期、病理类型以及ERCC1和TYMS的表达水平均是影响DFS的独立预后因素。结论ERCC1和TYMS表达与采用替吉奥联合顺铂治疗晚期非小细胞肺癌近期疗效与远期生存有相关性。

Objective To investigate the relationships between the expression of ERCC1 and TYMS and the curative effect of the S - 1 combined with platinum chemotherapy of non - small cell lung cancer patients and long - term survival. Methods Follow up of 68 cases received palliative resectio.n of lung cancer treatment, at the same time using S - 1 combined with cisplatin chem- otherapy in advanced （ ⅢB/Ⅳ ） in patients with non small cell lung cancer. Real -time PCR method was used to detect the ex- pression of tumor tissue ERCC1 and TYMS, Pearsonx2 test was used to analyze and compare the gene expression and the corre- sponding drug chemotherapy correlation, Cox regression analysis was used to screen independent prognostic risk factors. Results The efficiency of ERCC1 low expression group chemotherapy （ CR ＋ PR） was significantly higher than that in the high expression group （ P 〈 0.01 ） ; low expression of TYMS chemotherapy group effective rate was significantly higher than that of the high expression group （P 〈 0.01 ）. Multivariate Cox regression analysis showed that, the PS score, clinical stage, pathological type and the expression level of ERCC1 and TYMS were independent prognostic factors for DFS. Conclusion There is correla- tion among ERCC1 and TYMS expression and the short - term effects of S - 1 combined with cisplatin in treatment of advanced non small cell lung cancer as well as the long -term survival.