2013年北京市西城区沙门菌的监测、溯源及PFGE分型

Study on monitoring,roots and PFGE molecular typing of Salmonella in Xicheng district,Beijing in 2013

目的用脉冲场凝胶电泳(PFGE)分型技术,研究本区2013年肠道多病原监测标本中的沙门菌分子流行病学,查找沙门菌污染源,为预测疫情和制定防治措施提供依据。方法选择XbaⅠ酶对14株沙门菌全基因组DNA进行酶切,用PFGE技术对菌株进行分子分型,Bionumerisc统计软件聚类分析。用Kirby-Bauer法(KB)进行药敏试验,分析沙门菌耐药情况及同源性分析。结果沙门菌用XbaⅠ限制性酶切后,分成14个PFGE图谱类型。虽然表现出较大的遗传多样性,但部分菌株有相关性。14株受试菌株对绝大部分常用的头孢类抗生素敏感,但个别菌株对青霉素类、四环素、复方新诺明、庆大霉素产生了耐药。结论 PFGE分子分型与传统实验室检测技术紧密结合可增强沙门菌的溯源和预警。

Objective Using classification technology named pulsed field gel electrophoresis （PFGE） in 2013, the molecular types of Salmonella were studied of intestinal pathogen monitoring in this area. Finding Salmonella pollution sources, to predict outbreaks and provide evidence for prevention and control measures. Methods The 14 strains of Salmonella genome -wide DNA were digested by restriction endonuclease Xba I and analyzed by PFGE. The PFGE patterns of Salmonella strains from dif- ferent areas were compared using Bionumerisc statistical software to analyze the similarity between strains. The antibiotic suscep- tibility test was carried out using Kirby - Bauermethod （KB） to analyze the drug resistance pattern and homology. Results After Salmonella conducted by Xba I restriction enzyme digestion, there were 14 PFGE map types. Although it showed greater genet- ic diversity, some strains had correlation. More specifically, the 14 strains SalmoneUa were sensitive in most of the commonly used ccphalosporin＇ s antibiotics. But the individual strains of Salmonella had drug resistance to penicillin, tetracycline, paedia- tric compound sulfamethoxazole tablets and gentamicin. Conclusion The method of PFGE molecular classification integrated with the traditional technique of laboratory tests can strengthen the traceability and early warning of Salmonella.