2011年-2013年北京市严重急性呼吸道感染病毒病原学调查

Virus etiology investment on severe acute respiratory infection in Beijing from 2011 to 2013

目的了解北京地区严重急性呼吸道感染病毒病原学现况,探讨不同年龄组、不同月份病毒检出情况,为预防策略制定、临床诊断、治疗提供病原学依据。方法采用多重RT-PCR法,对270份咽拭子样本同时检测腺病毒,偏肺病毒,冠状病毒,1型、2型、3型、4型副流感病毒,甲型、乙型流感病毒,甲型、乙型呼吸道合胞病毒,甲型鼻病毒,博卡病毒和肠道病毒共14种常见呼吸道病毒。结果 270份样本中检出阳性样本103份,阳性率为38.15%。其中HBo V的检出率最高,为46.68%。15岁~25岁组呼吸道病毒检出阳性率最高,为66.67%。各病毒不同月份检出情况普遍呈10月-次年4月检出数增高,5月-9月有零星的散发。结论 HBo V和Flu为北京地区严重急性呼吸道感染主要致病原,随年龄不同、流行季节中月份不同而有一定的流行规律。

Objective To investigate the virus etiology situation on severe acute respiratory infection in Beijing and explore the detection rates of virus in different age groups or different months, so as to provide the data for the preventive strategy building, clinical diagnosis and treatment. Methods By muhiplex RT - PCR, adenovirus, metapneumovirus, coronavirus, parainfluenza virus 1, 2, 3, 4, influenza virus A, B, respiratory syncytial virus A, B, rhinovirus A, HBoV and EV 14 kinds of common re- spiratory viruses totally were detected in 270 clinical swabs simultaneously. Results 103 positive results were got from the 270 samples with the positive rate of 38.15%. In all the positive results, the HBoV was 46.48%, the highest. The detection rate in inpatients between 15 and 25 years old with respiratory viruses infection virus was found the highest of 66.67%. Every virus showed the higher positive rates from Oct. to next Apr. than that from May. to Sep. each year. Conclusion HBoV and Flu were the main pathogens leading to the acute upper respiratory infection in Beijing and the pathogens have specific epidemiology according to the ages and the seasons.