双酶分步水解鹅骨泥工艺的优化

Optimization of Stepwise Dual-enzyme Hydrolysis of Goose Bone Mud

利用酶法水解建立鹅骨泥酶解最优工艺条件。以鹅骨泥为原料,酶解液的水解度为指标,研究蛋白酶种类、酶解液pH值、酶解温度、鹅骨泥浓度和加酶量以及双酶分步水解对鹅骨泥酶解效果的影响。结果表明:中性-风味蛋白酶的双酶分步酶解效果更好,且加酶顺序对酶解有影响。酶解优化条件为50℃,总加酶量24000 U/g,时间比2∶2,双酶比例2∶2,酶解5 h后制备的酶解产物水解度可达44.94%。所得酶解液感官呈淡黄色透明、澄清液体,有淡淡骨香味,滋味丰富。

Establish the optimum enzymolysis process conditions of goose bone mud by enzymatic hydrolysis.Take goose bone mud as the raw material and hydrolysis degree of enzyme solution as the index,the single factor experiment is carried out to examine the effects on goose bone mud enzymolysis,including proteases, pH, hydrolysis temperature, substrate concentration, enzyme amount and stepwise dual-enzyme hydrolysis. The stepwise neutral-flavor protease dual-enzyme hydrolysis can get better result,and the enzyme sequence has an effect on the enzyme solution.The optimum condition is 50 ℃, the total amount of enzyme of 24000 U/g, time ratio of 2 ∶ 2, dual-enzyme ratio of 2∶2,hydrolysis time of 5 hours,and hydrolysis degree can reach 44.94%.The enzyme solution is clear liquid,with pale yellow transparent,delicate bone flavor and rich taste.