Aquaporin1在胶质瘤中的表达及其临床意义

Aquaporin1 expression in glioma patients and its potential function in glioma progression

目的:探讨水通道蛋白1(aquaporin1,AQP1)在胶质瘤中的表达情况及其与临床病理参数、预后的关系,观察AQP1对恶性胶质瘤细胞增殖、侵袭的影响。方法:采用免疫组织化学染色法检测135例肿瘤组织中AQP1的表达情况,分析AQP1与胶质瘤临床病理特征的关系,使用临床资料完整的103例胶质瘤标本分析AQP1与生存的关系。将AQP1连接至慢病毒表达质粒载体后感染恶性胶质瘤LN229细胞,Western blot检测目的蛋白的表达;MTT实验和Transwell小室实验分别检测胶质瘤细胞的增殖和侵袭能力。结果:AQP1的表达水平与胶质瘤的病理分级呈正相关,AQP1高表达与患者预后差相关(P<0.05)。过表达AQP1可增强胶质瘤细胞的增殖、侵袭能力(P<0.05)。结论:AQP1表达与胶质瘤进展密切相关,其表达上调可显著增强胶质瘤细胞的增殖和侵袭能力,有望为胶质瘤的临床检测及治疗提供新思路。

Objective：To explore the expression of aquaporin1 （AQP1） in human glioma tissues and its relationship with the clini-copathological parameters and prognosis of this tumor. This study also observed the function of AQP1 in the proliferation and invasion of LN229 glioblastoma cells. Methods：The expression of AQP1 in 135 cases of glioma was detected by immunohistochemical meth-od, and the correlation between AQP1 and pathological features of glioma was analyzed. The relationship of AQP1 with survival was al-so investigated using 103 specimens with complete clinical data. AQP1 was successfully transfected into LN229 cells with lentiviral vector, and the expression of AQP1 protein was tested by Western blot. Cell proliferation was detected by using methyl thiazolyl tetrazo-lium assay, whereas cell invasion was determined by Transwell assay. Results：The expression of AQP1 was positively correlated with pathological grading. High AQP1 expression was associated with poor prognosis （P〈0.05）. Moreover, the overexpression of AQP1 can significantly increase the proliferation and invasion of LN229 cells （P〈0.05）. Conclusion：AQP1 is closely associated with the progres-sion of glioma. Upregulation of the AQP1 expression promoted the proliferation and metastasis of glioma cells. These findings indicat-ed that AQP1 can function as a therapeutic target for glioma in future research.