Oka株带状疱疹减毒活疫苗猴体免疫原性分析

Immunogenicity of live attenuated herpes zoster vaccine prepared with Oka strain in rhesus monkeys

目的分析Oka株带状疱疹(herpes zoster,HZ)减毒活疫苗的免疫原性。方法分别用病毒滴度为5.46和4.9 lg PFU/ml的Oka株HZ减毒活疫苗,于恒河猴右侧上臂三角肌皮下进行免疫,观察免疫后恒河猴的一般状态,并于免疫后2、4、8、12周及6、9、12月采集静脉血,分离血清,荧光抗体膜抗原(fluorescent antibody to membrane antigen,FAMA)法检测血清中抗水痘-带状疱疹病毒(varicella-zoster virus,VZV)抗体水平。结果疫苗接种后无不良反应发生。免疫后2周,5.46和4.9 lg PFU/ml疫苗组抗体阳转率均为100%,几何平均滴度(GMT)分别为111.7和27.9;免疫后4周,GMT达到峰值,分别为168.8和84.4;免疫后12个月,抗VZV抗体滴度仍保持在较高水平。结论Oka株HZ减毒活疫苗具有良好的猴体免疫原性。

Objective To analyze the immunogenicity of live attenuated herpes zoster（HZ） vaccine prepared with Oka strain. Methods Rhesus monkeys were immunized s. c. with live attenuated HZ vaccine prepared with Oka strain, at virus titers of 5. 46 and 4. 9 lg PFU / ml respectively, and observed for general status, of which the serum samples were collected 2, 4, 8 and 12 weeks and 6, 9 and 12 months after immunization, and determined for antibody level against varicella-zoster virus（VZV）with fluorescent antibody to membrane antigen（FAMA）. Results No adverse reactions were observed after immunization. Both the antibody positive conversion rates of monkeys 2 weeks after immunization with the vaccine at virus titers of 5. 46 and 4. 9 lg PFU / ml were 100%, while the GMTs 2 weeks after immunization were 111. 7and 27. 9, and those 4 weeks after immunization reached peak values of 168. 8 and 84. 4, respectively. The antibody titer against VZV were still at high levels 12 months after immunization. Conclusion Live attenuated HZ vaccine prepared with Oka strain showed good immunogenicity in rhesus monkeys.