电针腧穴“胃病方”不同留针时间对急性胃黏膜损伤大鼠转化生长因子-α、热休克蛋白70的影响

Effect of duration of electroacupuncture at “Weibing Fang” on transforming growth factor-α and heat shock protein 70 expression in acute gastric mucosal injury in rats

目的:观察电针"胃病方"(足三里+中脘+内关)针刺留针不同时间对急性胃黏膜损伤大鼠胃黏膜的修复效应,并探讨其最佳留针时间及时效关系.方法:将80只大鼠随机分为空白组、模型组及30 s、3、5、10、15、30 min不同留针时间组,共8组.采用无水乙醇灌胃法制作模型,免疫组织化学法检测胃黏膜转化生长因子-α(transforming growth factor-α,TGF-α)和热休克蛋白70(heat shock protein 70,HSP70)的表达.结果:10、15、30 min针刺治疗组TGF-α表达的积分光密度值较模型组明显升高(P<0.05),且15、30 min治疗组较其余4治疗组差异具有统计学意义(P<0.05),其中15 min和30 min两组间比较,并无明显差异.各留针时间针刺组HSP70表达的积分光密度值较模型组明显升高(P<0.05).30 s、3、5 min 3组均与10、15、30 min比较,后3组积分光密度值明显高于前3组,差异具有统计学意义(P<0.05);10 min组与15、30 min比较,后两组HSP70表达更为明显(P<0.05).提示针刺可使急性胃黏膜损伤大鼠胃黏膜TGF-α和HSP70表达增强,但针刺长时间留针(15 min和30 min两组)更具有显著的效果.结论:长时间留针能更有效的修复大鼠胃黏膜损伤,其机制可能与促进内源性保护物质合成有关.

AIM： To assess the effect of needle retaining time of electroacupuncture at ＂Weibing Fang＂on the repair of acute gastric mucosal injury in order to explore the optimal needle retaining time and the time-effect relationship.METHODS： Eighty Wistar rats were randomly divided into 8 groups： a blank group, a model group, and six electroacupuncture treatment groups with different needleretaining times. Acute gastric mucosa injury was induced using anhydrous alcohol gavage.Immunohistochemistry assay was used for the detection of gastric mucosal transforming growth factor-α（TGF-α） and heat shock protein70（HSP70） expression.RESULTS： The integral optical density of TGF-α expression in the 10-, 15-, 30-min acupuncture groups was significantly higher than that in the model group（P 0.05）. The integral optical density of TGF-α expression in the 15- and 30-min groups were statistically different from that in the other 4 treatment groups（P 0.05）, although there was no significant difference between the 15- and30-min groups. The integral optical density of HSP70 expression in the acupuncture treatment groups was significantly higher than that in the model group（P 0.05）. The integral optical density of HSP70 expression in the30-s, 3-min and 5-min groups was significantly lower that in the 10, 15 and 30-min groups（P 0.05）; HSP70 expression in the 10-min group was significantly lower that in the15-min and 30-min groups（P 0.05）. Longduration（15 min and 30 min） acupuncture upregulated TGF-α and HSP70 expression more significantly compared with other groups.CONCLUSION： Long-duration acupuncture has better effect on rat gastric mucosal repair, and the mechanism may be related to promoting endogenous protective substance expression.