生物阳极及其反转为生物阴极降解氯霉素

Bioanode and Inversion of Bioanode to Biocathode for the Degradation of Antibiotic Chloramphenicol

为了探讨生物阳极能否降解氯霉素以及生物阳极反转为生物阴极后能否替代生物阴极，通过驯化生物阳极降解氯霉素和生物阳极反转为生物阴极的实验，表明生物阳极经过长期氯霉素浓度梯度增加（5～80mg·L^-1）的驯化，具有较好产电能力的同时，对氯霉素也具有一定的降解能力（k=0．0985）．生物阳极反转为生物阴极后并将电位恒定在－0．40V vs．SHE时，该生物阴极相对于反转前生物阳极的电位（－0．20VVS．SHE）有了明显的降低，导致微生物活性受到一定的影响，但阴极生物膜仍具有较强的催化降解氯霉素的能力，其还原降解速率常数k为0．2643，明显高于非生物阴极对照（k=0．1609）．生物阳极反转生物阴极的模式不仅实现了氯霉素的硝基还原，而且发生了芳香胺产物侧链的完全脱氯和羰基还原反应．

In order to investigate the possibility of the normal bioanode and bioanode switched to biocathode for the bio-electrochemical degradation of the antibiotic chloramphenicol （CAP）, both the bioanode acclimated with CAP and the biocathode inversed from bioanode wore monitored for CAP degradation in the bio-eloctrochemical system. The results demonstrated that the normal enriched bioanode could simultaneously generate current and degrade CAP （k = 0. 098 5, 35 mg·L^-1 of CAP） after a long-term acclimation by gradually increasing the concentration of CAP from 5 mg·L^-1 to 80 mg·L^-1. After switching bioanode to biocathode, the cathode biofilm was still capable of catalyzing CAP degradation, although it was influenced to some extent due to changed electrode potential from - 0.20 V to - 0.40 V vs. standard hydrogen electrode （ SHE）. The k of bioeathode was 0. 264 3, significantly higher than that of abiotic cathode （k = 0. 160 9）. This mode of biocathode, which was switched from bioanodc, not only had the ability of reducing nitro group in CAP but also catalyzed the complete dcchloridation and carbanyl group reduction of the side chain of aromatic amine product.