摘要
目的探索Vero细胞在无血清条件下制备乙型脑炎灭活疫苗的工艺。方法用无血清培养基培养Veto细胞,并将乙型脑炎病毒P3V2株毒种接种于培养的Vero细胞,观察细胞和病毒的生长情况。收获病毒液,通过灭活、纯化制备乙型脑炎灭活疫苗,检测疫苗各项指标,并与有血清培养工艺制备的疫苗(2013年生产的疫苗)进行比较。结果无血清培养基培养的Vero细胞生长良好,培养5d的细胞呈致密单层生长。3批无血清培养工艺制备的疫苗的第1、2和3次收获液的平均病毒滴度分别为8.847、8.319和7.194lgLD50/ml。3批无血清培养工艺制备的疫苗半成品的平均抗原含量(1.51Eu/ml)与有血清培养工艺制备的疫苗(1.41EU/ml)相当,但前者的宿主细胞蛋白含量(19.69μg/L)则明显低于后者(63.90btg/L)。结论乙型脑炎病毒可在无血清Vero细胞中良好生长,Vero细胞无血清培养工艺制备乙型脑炎灭活疫苗是可行的。
Objective To explore a preparation process of inactivated Japanese encephalitis (JE) vaccine with Vero cells cultured by serum-free medium. Methods Vero cells were cultured by serum-flee medium and JE virus P3V2 strains were inoculated in the cultured Vero cells. The growth of cells and viruses were observed. The virus was harvested, and inactivated JE vaccine was prepared after inactivation and purification of harvest. Various indicators of inactivated JE vaccine prepared by serumfree culture process were detected, and the detection results of inactivated JE vaccine prepared by serumfree culture process were compared with those of inactivated JE vaccine prepared by serum culture process. Results Vero cells cultured by serum-free medium grew well, reaching dense layer after 5 days' culture. The average virus titers of 3 batches of single harvested vaccine viruses were 8. 847 lgLD50/ml for the first harvest, 8. 319 lgLD50/ml for the second harvest and 7. 194 lgLD50/ml for the third harvest. The average antigen contents (1.51 EU/ml) of 3 hatches of final bulk of JE vaccine prepared by serum-free culture process were similar to those (1.41 EU/ml) of inactivated JE vaccine prepared by serum culture process, but the average contents (19. 69 μg/L) of host cell protein of the former were significantly lower than those (63.90μg/L) of the latter. Conclusion JE viruses grow well in Vero cells cultured by serum-free medium, and inactivated JE vaccine prepared by serum-free culture process is feasible.
出处
《国际生物制品学杂志》
CAS
2015年第3期112-115,120,共5页
International Journal of Biologicals
关键词
培养基
无血清
VERO细胞
脑炎病毒
日本
疫苗
灭活
Culture medium, serum-free
Vero cells
Encephalitis virus, Japanese
Vaccines,inactivated