复合益生菌与金霉素配伍抑制金黄色葡萄球菌生长的研究

The impact of the combined probiotics with chlortetracycline on inhibiting S.auresus proliferation

为降低抗生素的使用剂量,利用正交试验,研究四种益生菌与金霉素配伍对金黄色葡萄球菌生长的抑制作用。结果表明,复合益生菌抑制金黄色葡萄球菌的最佳组合为：枯草芽孢杆菌：酵母菌：乳酪杆菌：粪肠球菌=4：1：4：1；单独复合益生菌对金黄色葡萄球菌的抑制作用与200~300 mg/kg的金霉素相当。随着金霉素浓度的提高,无论是单一的金霉素或是其与益生菌的复合物,其抑菌作用逐渐增强（P〈0.05）。当150 mg/kg金霉素与益生菌配合后,其抑菌效果与单一（400~500 mg/kg）金霉素作用相当。由此可知,低浓度金霉素与复合益生菌配合后,可以达到高浓度金霉素的抑菌效果。

In order to reduce the use of antibiotic dose, orthogonal design was used to study the com- bined effect of probiotics and chlortetracycline on inhibiting S.auresus proliferation. The results showed that the optimal ratio of Bacillus subtilis, Saccharomyces cerevisiae boulardii, Lactobacillus ea- sel and Enterococcus faecalis was 4 ： 1 ： 4 ： 1, which had the same ability as 200-300 mg/kg chlor- tetracycline for inhibiting S.auresus proliferation（P〈0.05）. The ability of inhibiting S.auresus prolifera- tion with 150 mg/kg chlortetraeycline and probiotics was the same as the individual 400-500 mg/kg chlortetracycline, indicating that the low level of chlortetracycline with probiotics addition had the same ability as the individual high levels of chlortetracycline addition.