3种野生浆果果酒中活性物质及抗氧化活性比较

Comparison of antioxidant activity and active substance of three kinds wild berry wine

研究了白刺、沙棘、黑枸杞发酵果酒中黄酮、多酚、多糖、原花青素、花色苷的含量及其对铁离子的还原力和亚油酸脂质过氧化的抑制效果,采用DPPH、ABTS、H2O2/Fe2+/水杨酸、超氧阴离子自由基清除体系考察了3种果酒的抗氧化能力,并对活性成分含量与抗氧化活性进行了相关性分析。结果表明,沙棘酒中多糖含量最高,为0.605 mg/m L,其对·OH的清除率最高,为97.98%;黒枸杞酒中花色苷含量最高,为0.245 mg/m L,其对DPPH·的清除率最好,为97.14%,对铁离子的还原能力最高为11.29 mg/m L;白刺酒对O2-·的清除率最高为90.93%。对活性成分含量与抗氧化活性相关性分析表明,果酒对DPPH·的清除作用与酒中花色苷的含量呈正相关;果酒中多糖含量对·OH的清除呈正相关而对DPPH·的清除呈负相关;果酒中黄酮、多酚和原花青素含量与ABTS·及亚油酸过氧化的抑制呈正相关,与O2-·的清除率呈负相关。

The relationship between the active ingredient and antioxidant activity of three kinds wild berry wine from Nitraria tangutorum Bobr, Hippophae rhamnoides and Lycium ruthenicum Murr was studied. The three kinds of wild berry wines were evaluated based on their abilities to scavenge DPPH, ABTS, hydroxyl, and superoxide anion radicals, to reduce Fe2＋ , and to inhibit linoleic acid lipid peroxidation. Their correlation with the contents of flavone, polyphenols, polysaccharides, procyanidine and anthocyanin was analyzed. The results showed that sea buckthorn wine had strong scavenging activity against hydroxyl radicals with clearance rate of 97.98% and had the highest con- tent of polysaccharide of up to 0. 605 mg/mL. Black Chinese wolfberry had better scavenging activity of 97.14% a- gainst DPPH and reduction capacity of 11.29 mg/mL for iron ion, and its anthocyanins content was the highest, which was up to 0. 245 mg/mL. Nitraria wine had better scavenging activity against superoxide anion radicals with clearance rate of 90.93%. Correlation analysis showed that there were positively correlations between the anthocyanin contents of these three kinds of wild wines and their abilities to scavenge DPPH radicals. Meanwhile, polysaccharides contents of wild wines was positively correlated to their scavenging abilities against hydroxyl radicals, but was nega- tively correlated to their scavenging abilities against DPPH radicals. Moreover, our results suggested that flavone, polyphenols and polysaccharides contents in wild wines were positively correlated to their scavenging abilities against ABTS radicals, but were negatively correlated to their scavenging abilities against superoxide anion radicals.