HPLC法测定豆浆中的嘌呤含量

Determination of purine in soybean milk by HPLC

利用HPLC法对豆浆中的嘌呤物质进行检测分析,建立腺嘌呤、鸟嘌呤、黄嘌呤和次黄嘌呤4种嘌呤物质的多组分检测条件并进行方法评价。研究表明:利用Zorbax C18反相色谱柱(4.6 mm×250 mm,5.0μm),流动相以KH2PO4缓冲液(0.02 mol/L,p H 3.0),在流速1.0 m L/min,柱温25℃,进样量10μL,检测波长(λ1)为254 nm的条件下,豆浆中的4种嘌呤达到最好分离,其精密度和回收率小于2%。

A feasible method using HPLC which was evaluated for analyzing Adenine, Guanine, Xanthine, Hypoxanthine from soybean milk was established in this paper. The chromatographic conditions were optimized as follows： Zorbax C18 column （4.6 mm ×250 mm, i. d. 5.0 μm） , 254 nm by UV detector with 0.02 mol/L of KH2PO4 buffer solution （pH = 3.0） as mobile phase, the flow rate was 1.0 mL/min, the column temperature was 25 ℃, and the sample size was 10 μL. The result showed that several purines can be separated effectively in these conditions with the precision and recovery under 2%.