广州市海珠区723例疑似登革热病例血清学检测分析

Results of serological detection of 723 suspected cases of dengue fever in Haizhu district of Guangzhou

目的分析疑似登革热病例实验室Ig M抗体检测结果,为有效控制登革热流行提供科学依据。方法收集临床医生报告的疑似登革热病例血清样本,随机采用金标免疫层析法（ICT）或捕捉酶联免疫吸附试验（Mac-ELISA）进行病毒特异性Ig M抗体检测,统计学比较两种检测方法的差异,分析区域内登革热流行特征。结果共检测样本723例,其中ICT法检测样本345例,检出阳性100例,阳性检出率为28.99%;Mac-ELISA法累计检测样本486例,阳性检出120例,阳性检出率24.69%,两种方法的阳性检出率差异无统计学意义（χ2=1.911,P〉0.05）。全区18条街道均有阳性病例发生,发病高峰期为10月,20~49岁年龄段为主要发病人群,男女性别比为1：1.12。结论区域内登革热发病集中在人口密集及卫生环境相对较差的区域,阳性病例集中出现在8~11月,中青年为主要发病人群,应适时防控,严防暴发流行;实验室检测结果与采样时间密切相关,ICT检测方法可以作为登革热流行期间早期筛查,及时采集患者血清进行Ig M抗体检测对疫情防控具有重要意义。

Objective To analyze the results, of detection of dengue IgM antibody in suspected dengue fever cases in order to provide data for control of dengue fever. Methods Serum samples were collected from the patients with suspected dengue fever reported by clinicians, ICT or ELISA assay was used to randomly detect the specific dengue IgM antibody, the results of detection by the two methods were compared, and the epidemiological characteristics of dengue fever in Haizhu district of Guangzhou was analyzed. Results IgM antibody in 723 serum samples were tested, 345 samples were tested by ICT and 100 sample were positive with the positive rate of 28.99%,while 486 samples were tested by ELISA and the positive rate was 24.69% without significant differences between these two assays （χ2=1.911, P〉0.05）.Dengue fever cases were found in all the 18 lanes.The peak of onset was in October and infection mainly concentrated in age group of 20-49 years with the male to female ratio of 1：1.12.Conciusion Dengue fever mainly occurred in densely populated and poorly hygienic areas. Positive cases were mostly detected from August to November concentrated in young and middle aged people. Strategies should be taken in time to prevent dengue fever outbreak and epidemic. ICT could be used for early screening of dengue fever infection and serological detection of specific IgM antibody on time is fundamentally important for controlling the epidemic of dengue fever.