miR-21联合CEA在结肠癌诊断中的应用

Application of miR-21 combined with CEA in the diagnosis of colorectal cancer

目的探索mi R-21联合癌胚抗原(CEA)对结肠癌的诊断意义,为结肠癌早期检测提供新的依据。方法选择2010年12月至2013年12月于收治的结肠癌患者92例,匹配正常人88例;采集一般信息包括年龄、性别和CEA表达情况等,提取血浆中RNA并用Q-PCR检测mi R-21的表达情况;分别用ROC曲线检测CEA、mi R-21、mi R-21联合CEA对结肠癌的诊断价值。结果正常人组CEA水平为(1.793±1.121)ng/m L,结肠癌患者CEA水平为(9.843±8.430)ng/m L,两组比较差异具有统计学意义(P<0.05)。Q-PCR结果显示结肠癌患者mi R-21表达水平是正常人的(5.510±3.971)倍,差异具有统计学意义(P<0.05)。ROC曲线分析结果显示,CEA组曲线下面积为(0.838±0.029)(95%CI 0.781-0.894),mi R-21组曲线下面积为(0.724±0.038)(95%CI 0.649-0.798),mi R-21联合CEA组曲线下面积(0.916±0.019)(95%CI0.878-0.953)。结论 mi R-21联合CEA优于CEA或mi R-21单独对于结肠癌的诊断价值,为临床早期检测结肠癌提供了新的依据。

Objective To explore the value of combined detection of mi R-21 and CEA in diagnosis of colorectal cancerMethods Ninety- two colorectal cancer patients and 88 normal subjectss in Surgery Department of Affiliated Hospital ofHainan Medical College were chosen from December 2010 to December 2013; Basic conditions of the cases and subjects（age,gender and CEA level）were collected; Q-PCR were used to detect the expression of mi R-21; The curve of ROC was used toanalyze the predictive value of CEA, mi R-21, mi R-21 combined with CEA.Results The CEA levels in normal group andcolorectal cancer patients were（1.793±1.121）ng /m L and（9.843±8.430）ng /m L, the difference was statistically significant. Q-PCR results showed that expression level of mi R-21 in colorectal cancer patients was（5.510±3.971）times of that in normalsubjects, the difference was statistically significant. The areas under the ROC curve of CEA, mi R-21, mi R-21 combined withCEA were（0.838±0.029）（95%CI 0.781-0.894）,（0.724±0.038）（95% CI 0.649-0.798）and（0.916±0.019）（95%CI 0.878-0.953）.Conclusion Combined detection of CEA and mi R- 21 is superior to that of detectiing CEA or mi R- 21 alone indiagnosis of colorectal cancer as it provides new basis for early clinical detection of colorectal cancer.