免疫鼠抗AT_1受体自身抗体的类型分布研究

Study on Distribution of the Types of Autoantibodies against AT_1 Receptor in the Sera of Immunized Rats

目的:明确免疫鼠血中抗AT1受体自身抗体的类型分布,为该抗体的作用机制研究提供一定的实验依据。方法:以AT1R-ECII为抗原主动免疫雌性Wistar大鼠,待大鼠血清中含有高滴度的抗AT1受体自身抗体后,用ELISA法检测血清中抗AT1受体抗体的水平、类型及亚类。结果:雌性Wistar大鼠免疫4周后,抗AT1受体抗体滴度时明显升高,8周时处于稳定高峰值(免疫组OD值2.99±0.11 vs.同期对照组0.44±0.06,P<0.05);该抗体属于免疫球蛋白的Ig G类;在大鼠Ig G的4个亚型(Ig G1,Ig G2a,Ig G2b,Ig G2c)中,该抗体大部分属于Ig G2b亚类(OD值为0.33±0.04 vs.同期对照组OD值0.08±0.01,P<0.05),极少部分属于Ig G2a亚型(OD值为0.10±0.01 vs.同期对照组OD值0.07±0.05,P<0.05)。结论:通过主动免疫大鼠体内产生的抗AT1受体抗体主要属于Ig G类,Ig G2b亚类。

Objective： To detect distribution of the types of autoantibodies against AT1 receptor in the sera of immunized rats, providing the experimental basis for the research of the action mechanism. Methods： Adult female Wistar rats were actively immunized with AT1R-ECII. When the titer of anti-AT1R antibody reached a peak, the titers, types and subtypes of anti-AT1R antibody in the sera of rats were detected by SA-ELISA. Results： The titers of serum anti- AT1R antibody in immunized group were increased at the 4th week and attained a peak value at the 8th week as compared with the control group at the same time point （ OD value, 2.99 ±0.11 vs. 0.44 ± 0.06, P 〈 0.05 ）. It was shown that anti-AT1R antibody was IgG type immunoglobulin（ IgG1, IgG2a, IgG2b, IgG2c）. Meanwhile, the antibody was mainly determined as IgG2b subtype （ OD value, 0. 33 ± 0.04 vs. 0.08 ± 0.01, P 〈 0.05 ）, and a few belonged to IgG2a subtype（ OD value, 0.10± 0.01 vs. 0.07 ±0.01, P 〈 0.05 ）. Conclusion ：The anti-AT1 R antibodies in immunized rats aremainly determined as IgG2b subtype, and a few belongs to IgG2a subtype.