七氟醚预处理对大鼠心肌缺血再灌注的影响与Caveo-lin-3表达的关系

Effects of Sevoflurane Preconditioning on Myocardial Cells during Myocardial Ischemia-reperfusion in Rats and the Relationship of the Expression of Caveolin-3

目的:评价不同浓度及不同给药模式七氟醚预处理对大鼠心肌缺血再灌注的影响及Caveolin-3在其中的作用。方法:采集健康雄性Wistar大鼠,使用改良Langedorff灌注装置制备离体心脏灌注模型,将其均分为6组(n=10):对照组(C组)、缺血再灌注组(IR组)、持续1.5%七氟醚预处理组(APC-1)、持续2.5%七氟醚预处理组(APC-2)、间断1.5%七氟醚预处理组(APCI-1)和间断2.5%七氟醚预处理组(APCI-2)。采用氯化三苯基四氮唑染色法评价离体心脏缺血再灌注损伤梗死面积,取左心室心肌细胞测定Caveolin-3的表达。结果:与对照组相比,缺血再灌注组心肌梗死面积增大,Caveolin-3表达下降,差异有统计学意义(P<0.05)。与缺血再灌注组相比,七氟醚预处理组心肌梗死面积减小,Caveolin-3表达上调,差异有统计学意义(P<0.05)。不同浓度及给药模式的七氟醚预处理组间相比,持续给药模式较间断给药模式组心肌缺血再灌注梗死面积减小,Caveolin-3表达上调最明显,差别有统计学意义(P<0.05),而不同浓度七氟醚组间心肌梗死面积、Caveolin-3表达无统计学意义(P>0.05)。结论:七氟醚预处理能减轻大鼠心肌缺血再灌注损伤,缩小梗死面积;持续七氟醚预处理具有明显的心肌保护作用其作用机制可能与上调Caveolin-3的表达有关。

Objective： To evaluate the effects of sevoflurane preconditioning with different concentration and different administration model on myocardial cells during myocardial ischemia-reperfusion in rats and the role of Caveolin-3. Methods：The healthy male SD rats were completely randomly assigned to 6 different groups （n = 10 each） using a random number table, controlling group （ group C）, I/R group, Lasting 1.5 % sevoflurane preconditioning group （ APC-1 ）, Lasting 2.5% sevoflurane preconditioning group （APC-2）, Intermittent 1.5% sevoflurane preconditioning group （APCI-1 ） and intermittent 2.5% sevoflurane preconditioning group （APCI-2）. TFC （Triphenyl tetrazolium chloride） staining e- valuated the infarct size of myocardial infarction, and immunohistochemistry evaluated the expression of Caveolin-3 on left ventricle. Results： Compared with the controlling group （group C）, myocardial infarction area increased and Caveolin-3 expression decreased in group I/R. There was statistically significant difference （P 〈0.05 ）. Compared with the group I/R, myocardial infarction area decreased, and Caveolin-3 expression raised in Sevoflurane preconditioning group. There was statistically significant difference （P 〈 0.05 ）. In different sevoflurane preconditioning group, myocardial infarction size decreased, and Caveolin-3 expression raised. There was statistically significant difference in last sevoflurane preconditioning group compared with intermittent sevoflurane preconditioning group （P 〈 0.05）. But myocardial infarction area and Caveolin-3 expression had no statistical significance in different concentrations of sevoflurane group （P 〉 0.05）. Conclusion： Sevofiurane preconditioning can attenuate myocardial ischemia reperfusion injury in rats, possibly associated with upregulated Caveolin-3 expression and narrowing the area of myocardial infarction. The effection of myocardial protection is the most obvious in continuing sevoflurane preconditioning group.