玉米芯诱导里氏木霉Rut C30产纤维素酶的分析

Analysis of Cellulase Production with Trichoderma reesei Rut C30 Induced by Corn Cob

分别以不同木质纤维素类为碳源(质量浓度均为10,g/L)诱导里氏木霉Rut C30产酶,通过测定诱导过程的产酶曲线,结果表明:以玉米芯为诱导碳源时,各酶组分的产酶水平最高,诱导120,h后纤维素酶滤纸酶活、β–葡萄糖苷酶和木聚糖酶酶活分别为1.53,FPU/m L、0.61,IU/m L和72.86,IU/m L.采用高效液相色谱(HPLC)法分析了玉米芯水解过程中糖的组成及变化,可检测到的单糖包括葡萄糖、木糖、阿拉伯糖、甘露糖和半乳糖以及纤维二糖等寡糖.诱导产酶实验表明单糖中只有半乳糖可诱导纤维素酶的合成.

Trichoderma reesei Rut C30 was induced by different lignocellulosic inducers with the same concentration of 10 g/L to produce enzymes, and the enzyme-producing curves were determined. Results indicated that the production of cellulase, β-glucosidase and xylanase were the highest when corn cob was used as the inducing carbon source, and the production was up to 1.53 FPU/mL, 0.61 IU/mL and 72.86 IU/mL respectively after inducing 120 hours. The sugar composition and variation during the enzymatic hydrolysis of corn cob were analyzed with high performance liquid chromatography （HPLC）. Monosaccharide detected included glucose, xylose, arabinose, mannose and galactose. The results of induction experiments indicated that only galactose among monosaccharides could induce cellulase.