鱼腥草素钠抗类风湿关节炎滑膜增殖作用的实验研究

Inhibition of sodium houttuyfonate on synovial proliferation in rats with rheumatoid arthritis

目的研究鱼腥草素钠(SH)抗类风湿关节炎(RA)的滑膜增殖作用。方法 Wistar大鼠随机分成5组:对照组、模型组、SH 130 mg·kg-1组、SH 260 mg·kg-1组、SH 520 mg·kg-1组。建立大鼠胶原诱导的类风湿关节炎(CIA)模型,计数方法测定大鼠关节炎指数(AI),称重法比较大鼠的体重变化,HE染色法光镜观察关节病理形态学变化,酶联免疫法(ELISA)检测血清中细胞因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、血管内皮生长因子(VEGF)、PKA、c AMP水平及免疫组化检测滑膜细胞凋亡情况。结果SH干预组关节炎指数较模型组低,体重降低不明显,关节破坏较模型组轻,细胞因子水平较模型组明显减低,滑膜细胞凋亡较模型组明显(P<0.01)。结论 SH可以增加RA滑膜细胞的凋亡,降低CIA大鼠的AI及炎症因子的水平。

Objective To investigate the inhibition of sodium houttuyfonate on synovial proliferation in rats with rheumatoid arthritis. Methods Totally 50 Wistar rats were randomly divided into 5 groups： a control group （group 1）, a model group （group 2）, a 130 mg kg - 1 SH-treated group （group 3）, a 260 mg kg - 1 SH-treated group （group 4）, and a 520 mg kg -1 SH-treated group （group 5）. Type U collagen and complete Freund＇ s adjuvant （CFA） were injected into the rats to create the collagen induced arthritis （CIA） model. Arthritis index （AI） and weight were used to evaluate the inflammatory reaction in the rats. The levels of IL-6, TNF-a and VEGF, PKA, cAMP were measured by enzyme-linked immunosorbent assay （ELISA）. The synovicytes apoptosis was detected by immunohistochemistry. Results The AI was lower in the SH-treated groups than that of the model group. Similar results were shown in the weight variability, joints＇ destruction, levels of cytokines and the synovicytes apoptosis between the SH-treated groups and the model group. Conclusion SH can enhance synovicytes apoptosis in rats with RA and reduce the AI and the levels of inflammatory factors.