冬凌草甲素对人肝癌细胞HepG_2缝隙连接功能的影响

Effects of oridonin on HepG_2 in human

目的：观察冬凌草甲素对人肝癌细胞Hep G2缝隙连接功能（GJIC）的影响。方法：采用划痕标记染料示踪技术（Scrape-loading Dye Transfer Assay,SL/DT）,观察不同浓度的冬凌草甲素处理人肝癌细胞Hep G2之后细胞间隙连接通讯功能。结果：空白对照组细胞的荧光染料主要出现在划痕旁1-2列细胞中（±）,且划痕两边被染色的细胞较少;细胞经冬凌草甲素处理48h后,荧光染料不仅出现在划痕的附近细胞内,而且在划痕以外的3~4列细胞内也出现了荧光（＋＋＋＋）,划痕两边被染色的细胞明显增多,出现了明显的细胞间染料传输现象,呈浓度依赖性。结论：冬凌草甲素能增强Hep G2细胞间缝隙连接功能,可能是其抗肿瘤作用机制之一。

Objective： To observe effects of oridonin on HepG2. Methods： After dispose HepG2 with different concentrations of oridonin, the HepG2 function of gap junctional intercellular communication was observed by SL/DT. Results： Fluorochrome of the untreated control group mainly occurred in the cells which were located 1-2 row （-/-） beside scratches and had less dyed cells in the scratches on both sides. Cells treated with oridonin treatment after 48 hours, fluorochrome mainly occurred in the cells which were located beside scratches and 3-4 row （ ＋＋＋＋ ） other than scratches. Dyed cells increased in the scratches on both sides, appeared intracellular dye transfer phenomenon and appeared a concentration dependent manner. Conclusion： Oridonin can enhance the function of HepG2 gap junction intercellular and may be one of antitumor mechanism.