吲哚-3-甲醇对人喉癌Hep-2细胞增殖的抑制作用研究

Study on the mechanism of Hep-2 cells proliferation inhibition induced by indoel-3-carbinol

目的研究吲哚-3-甲醇对人喉癌上皮细胞(Hep-2)增殖及凋亡的影响。方法实验分为5组,4个浓度实验组(吲哚-3-甲醇,0,50,100,150μmol·L-1)与对照组(二甲基亚砜)。用水溶性四氮唑法检测吲哚-3-甲醇对Hep-2细胞增殖的影响,用Hochest33258染色及流式细胞仪检测吲哚-3-甲醇诱导的Hep-2细胞的凋亡,用蛋白免疫印迹方法检测凋亡相关信号分子livin蛋白表达情况。结果经4个浓度实验组及对照组处理48 h后,Hep-2细胞的凋亡率分别为(3.95±0.68)%,(8.54±1.35)%,(21.28±2.32)%,(38.65±4.85)%和(4.26±0.58)%。表明吲哚-3-甲醇以浓度依赖方式抑制Hep-2增殖,诱导细胞凋亡和Livin蛋白表达。结论吲哚-3-甲醇可抑制Hep-2细胞增殖,其诱导细胞凋亡可能与Livin蛋白表达的抑制相关。

Objective To study the effect of indoel -3 -carbinol on Hep-2 cells in vitro.Methods There was divided into 5 groups, the 4 test groups （the concerntration of indoel-3-carbinol, 0, 50, 100, 150μmol＆#183; L-1） and the control group（dimethyl sulfoxide）.The inhibition of cell proliferation was assayed by method of WST-1.And the apopto-sis was shown with Hochest33258 staining and flow cytometry.The mechanism of apoptosis, and the expressions of the livin protein were detected by Western blotting assay.Results After being disposed by the test groups with 4 different concerntration of indoel-3-carbinol and the control group, the apoptosis rate of Hep-2 cells were（3.95 ±0.68）%, （ 8.54 ±1.35 ）%, （ 21.28 ±2.32 ）%, （ 38.65 ±4.85 ）%, and （4.26 ±0.58）%, respectively.The indoel -3 -carbinol could inhibit the proliferation of Hep-2 cells with the dose-dependent manner and the apoptosis could be induced too.Furthermore, the expressions of Livin appeared to be decreased significantly as compared with the control group.Conclusion The indoel-3-carbinol could induce apoptosis of Hep-2 cells in the manner of dose-dependent, and Livin might play a role during above processes.