双波长HPLC同时测定金刚藤软胶囊中5个活性成分的含量

Simultaneous Determination of Five Effective Constituents in Jingangteng Soft Capsule by Dual-wavelength HPLC

目的：建立双波长HPLC同时测定金刚藤软胶囊中绿原酸、白藜芦醇、芦丁、槲皮素和山柰酚含量的方法。方法：采用Agilent ZORBAX SB-C18色谱柱（4.6 mm×150 mm,5μm）,流动相乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 m L·min^-1,柱温25℃,检测波长306 nm（绿原酸、白藜芦醇）和365 nm（芦丁、槲皮素、山柰酚）。结果：绿原酸、白藜芦醇、芦丁、槲皮素、山柰酚分别在0.058 5~2.34μg（r=0.999 8）,0.025 3~1.01μg（r=0.999 9）,0.042 2~0.844μg（r=0.999 6）,0.018 1~0.722μg（r=0.999 9）,0.0165~0.660μg（r=0.999 9）呈现良好的线性关系,平均回收率分别为98.0%,100.6%,96.0%,102.7%,98.5%,RSD分别为1.2%,2.2%,1.8%,2.9%,1.3%。结论：该方法操作简便、重复性好,可作为金刚藤软胶囊中5个活性成分的含量测定方法。

Objective： To establish a dual-wavelength HPLC method for simultaneous determination of five constituents （chlorogenic acid, resveratrol, rutin, quercetin, kaempferol） in Jingangteng soft capsule. Method： The Agilent ZORBAX SB-Cls column （4.6 mm× 150 mm, 5μm） with a column temperature of 25℃ was used; the mobile phase was acetonitrile-0. 1% phosphoric acid at gradient elution program and with a flow rate of 1.0 mL· min^-1, and the detection wavelengths were set at 306 nm （ chlorogenic acid and resveratrol） and 365 nm （rutin, quercetin and kaempferol）. Result： The linear response ranges of chlorogenic acid, resveratrol, rutin, quercetin, and kaempferol were 0. 058 5-2.34μg （r = 0. 999 8） , 0. 025 3-1.01μg （r = 0. 999 9） , 0. 042 2- 0.844 μg （r = 0.999 6）, 0.018 1-0.722μg （r = 0.999 9）, and 0.016 5-0. 660 μg （r = 0.999 9）, respectively. The average recoveries were 98.0% , 100.6% , 96.0% , 102.7% , and 98.5% with RSDs of 1.2% , 2.2% , 1.8% , 2.9% , and 1.3% , respectively. Conclusion： The developed method was convenient and reliable, which could be applied to determination of five effective constituents in Jinggangteng soft capsule.