HS-SPME-GC-MS测定中药半枝莲中的挥发性成分

Analysis of the Volatile Compounds in Scutellaria barbata Using HS-SPME-GC-MS

目的:分析比较不同产地半枝莲的挥发性成分。方法:采用PDMS/DVB SPME萃取头,与气相色谱-质谱联用,分析江苏、广西南岭、江西南昌、湖南、四川5个产地半枝莲的挥发性成分,用归一化法计算各成分的相对含量,比较不同产地半枝莲含挥发性成分的差异。结果:从江苏、广西南岭、江西南昌、湖南、四川5个产地的半枝莲中共鉴定出挥发性化合物45个,共有化合物13个,分别为1-辛烯-3-醇,3,7-二甲基-1,6-辛二烯-3-醇甲酸酯,苯乙醇,甘菊环,2-甲氧基-3-(2-丙烯)苯酚,1,2-二甲氧基-4-(2-丙烯)苯,十五烷,2-甲基-,1-(1,1-二甲基乙基)-2-甲基-1,3-丙二醇丙酸酯,正十六烷,6,10,14-三甲基-2-十五烷酮,十六酸甲酯,邻苯二甲酸二丁酯,十六烷酸。结论:这13个共有峰可作为半枝莲挥发性成分中特征性指标成分,但不同产地半枝莲所含挥发性主成分有较大差异。

Objective： To analyze the volatile compounds in Scutellaria barbara froltl different places. Method： A headspaee solid-phase microextraetion （HS-SPME） method followed by gas chromatography-mass spectrometry （GC-MS） is described for the analysis of volatile compounds in S. barbata from Jiangsu, Guangxi, Jiangxi, Hunan and Sichuan provinces. The volatile compounds were extracted by PDMS/DVB SPME fiber. The relative content of each component was calculated by the normalization method. Result： There are 45 compounds identified in total and 13 volatile compounds in common from the five places mentioned above. The 13 volatile compounds are 1-octen-3-ol, 3, 7-dimethyl-1, 6-oetadien-3-ol methyl ester, phenylethyl alcohol, azulene, 2- methoxy-3- （2-propenyl） -phenol, 1 , 2-dimethoxy-4- （2-propenyl） -benzene, pentadecane, propanoic acid, 2-methyl-, 1- （1, 1-dimethylethyl） -2-methyl-I, 3-propanediyl ester, hexadecane, 6, 10, 14-trimethyl-2- pentadecanone, hexadecanoic acid methyl ester, dibutyl phthalate, n-hexadecanoic acid. Conclusion： The 13 volatile compounds were identified as the marker components in S. barbara. The results also suggest that there are some differences in the volatile compounds in S. barbata from different places.