三尖杉内生真菌次生代谢产物的活性成分分析

Active Constitunents Analysis of Secondary Metabolites in Endophytic Fungus from Cephalotaxus fortunei Root

目的:研究三尖杉内生真菌次生代谢产物的活性成分。方法:采用滤纸片扩散法和噻唑蓝比色法对三尖杉内生真菌CEP18发酵液的乙酸乙酯提取物进行抑菌和细胞毒活性测试,利用GC-MS分析活性成分。结果:三尖杉内生真菌次生代谢产物对枯草芽孢杆菌、金黄色葡萄球菌及人早幼粒急性白血病细胞HL-60的生长抑制作用明显。乙酸乙酯提取物中组分Fr2活性最强,主要成分为乳酸丁酯(2.96%),2-氯杀鼠灵酮(8.65%),(Z)-11-二十烯酰胺(13.90%),1-甲基芘(3.51%)及丁二酸二甲酯(6.34%)、对羟基苯乙酸(8.27%)、反油酸甲酯(10.07%)、亚油酸(5.60%)、棕榈酸(7.54%)。结论:对羟基苯乙酸在三尖杉属植物的相关研究中属首次报道,为微生物药物的研发和三尖杉的资源利用提供参考。

Objective： To study active constitunents of secondary metabolites in endophytic fungus from Cephalotaxus fortunei root. Method ： Antibacterial and antitumor activity of ethyl acetate extracts from fermentation liquor of an endophytie fungus （CEP18） in C. fortunei root were tested by filter paper diffusion method and MTT, active ingredients were analyzed by GC-MS. Result： Secondary metabolites in endophytic fungus from C. fortunei root significantly inhibited growth of Bacillus subtills, Staphylococcus aureus and Human promyelocytic leukemia cells （HL-60）. After silica gel column chromatography analysis and activity test, five components （Frl-Fr5） were isolated from ethyl acetate extracts, the Fr2 was identified as active component, which was mainly composed of butyl lactate （2.96%） , 2-chloro ketone warfarin （ 8.65% ） , （Z） -11-eieosenamide （ 13.90% ） , 1-methyl pyrene （3.51%） , dimethyl succinate （6.34%） , 4-hydroxyphenylacetic acid （8.27%） , elaidic acid-methyl ester （ 10.07% ） , linoleic acid （ 5.60% ） and hexadecanoic acid （ 7.54% ）. Conclusion： 4- Hydroxyphenylacetic acid is reported for the first time in C. genus at present research and is worthy of further development and utilization.