保肝颗粒对小鼠急性酒精性肝损伤的保护作用

Protective Effects of Baogan Granules on Acute Alcohol-induced Liver Injury in Mice

目的:研究保肝颗粒对小鼠急性酒精性肝损伤的保护作用。方法:将实验小鼠随机分为正常组、模型组、护肝片组、力克组、保肝颗粒高、中、低剂量组。护肝片组ig给予0.7 g·kg-1·d-1,力克组为1.18 g·kg-1·d-1,保肝颗粒高、中、低分别为12.9,6.45,3.225 g·kg-1·d-1,正常组和模型组ig给予等体积的生理盐水。每天分别ig给药1次,连续30 d,末次给药1 h后,除正常组外,每组分别ig 50%乙醇0.012 m L·g-1,造成急性肝损伤模型,禁食不禁水24 h之后,杀鼠取材,检测小鼠血清丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST),总胆红素(TBIL),甘油三酯(TG),高密度脂蛋白(HDL),低密度脂蛋白(LDL)及肝脏组织石蜡切片HE染色观察组织病理学改变。结果:与正常组比较,模型组ALT,AST,TBIL,TG和LDL水平明显升高(P<0.05,P<0.01),HDL含量明显降低(P<0.01);与模型组比较,保肝颗粒高、中、低剂量组血清ALT,AST,TBIL,TG,LDL水平明显降低(P<0.01,P<0.05),HDL水平升高(P<0.01,P<0.05)。病理观察显示,保肝颗粒高、中、低剂量组肝细胞的形态、结构、水肿和炎性浸润有不同程度的改善。结论:保肝颗粒对小鼠血清中ALT,AST,TG和LDL的含量抑制明显,但对TBIL的含量抑制不明确,能有效促进HDL生成,改善肝细胞水肿及炎性浸润,从而能达到对酒精性肝损伤的保护。

Objective： To observe the protective effect of Baogan granules liver injury in mice. Method： The experimental mice were randomly divided into group, the Hugan tablet group （0.7 g·kg·d^-1 ） , the Like group （1. 18 g·kg^-1.d low-dose Baogan granules groups （12.9, 6.45, 3. 225 g·kg^-1·d^-1 ）. The mice in on acute alcoholic-induced e normal group, the model , the high-, medium-, and e normal and model groups received an equal volume of saline. All mice received 30-day oral administration of the corresponding medicines. The acute liver injury model was induced in mice by feeding 50% alcohol at 0. 012 mL·g^-1 h later after the last administration. The serum levels of alanine aminotransferase （ALT） , aspartic transaminase （AST） , total bilirubin （TBIL）, triglyceride （TG）, high-density lipoprotein （HDL） and low-density lipoprotein （LDL） were detected. Histopathologieal changes of liver tissue were observed by using the HE staining. Result ： Compared with the model control group, the levels of ALT, AST, TBIL, TG and LDL increased, the level of HDL decreased in the model group （P 〈 0.05, P 〈 0.01 ）. Compared with model group, the levels of ALT, AST, TBIL, TG and LDL decreased, the level of HDL increased in the high-, medium-, and low-dose Baogan granules groups （P 〈 0.05, P〈0.01 ）. Pathological results showed that the liver cell morphology, structure, edema and inflammatory infiltration had different degrees of improvement in the Baogan granules groups. Conclusion： Baogan granules could obviously inhibit the serum levels of ALT, AST, TG and LDL; promote the HDL generation in mice. However, its effect on TBI is unclear. Moreover, it could protect the alcoholic-induced liver injury by improving liver edema and inflammatory cell infiltration.