肝癌患者外周血CD25及其mRNA表达

Expression of CD25 and its m RNA in peripheral blood of patients with hepatocellular carcinoma

目的探讨肝癌患者外周血CD25及其m RNA表达。方法采用生物素-链霉亲和素(BSA)法检测患者外周血CD25水平,Real-time检测患者外周血CD25 m RNA含量。以3-磷酸甘油醛脱氢酶(GAPDH)为参照,以lgc DNA/lg GAPDH比值代表m RNA水平。结果肝癌患者外周血植物血凝素(PHA)诱导前后CD25表达水平分别为(2.54±1.15)%和(24.39±3.00)%,其m RNA表达水平分别为(1.56±0.24)lgc DNA/lg GAPDH和(2.93±0.43)lgc DNA/lg GAPDH,与正常对照组相比,差异有统计学意义(P<0.01);巨块型、结节型、弥漫型肝癌患者外周血CD25及其m RNA表达类似,差异无统计学意义(P>0.05)。18例患者以CIK(cytokine-induced killer)细胞治疗后,其静息期和诱导期CD25、CD25m RNA表达水平均明显升高,差异均有统计学意义(P<0.01)。结论肝癌患者细胞免疫功能明显低下,外周血CD25及其m RNA水平降低。CD25及其m RNA表达与病理分型无关,CD25似可作为观察患者细胞免疫功能和评估疗效的辅助指标。CIK细胞可明显上调肝癌患者外周血CD25表达。

[ Objective ] To study the expression of CD25 in serum and its mRNA in peripheral blood mononuc- lear cells （PBMCs） of patients with hepatocellular carcinoma. [ Methods ] The level of CD25 was detected by biotin- streptavidin （BSA）. The mRNA expression of CD25 in PBMCs was examined by real-time PCR. The glyceraldehyde- 3-phosphate dehydrogenase （GAPDH） was commonly used as internal control standard. The ratio of lgcDNA/ lgGAPDH was regarded as the extreme level of CD25 mRNA. [ Results ] The levels of CD25 and its mRNA in the peripheral blood of the patients before and after phytohemagglutinin （PHA） inducement were （2.54 ± 1.15）%, （24.39 ± 3.00）%, （1.56 ±0.24） lgcDNA/lgGAPDH, （2.93 ±0.43） lgcDNA/lgGAPDH, which were significantly lower than those in the controls （P 〈 0.01）. However, the similar expressions of CD25 and its mRNA were explored in hepato- cellular carcinomas of bulky, nodular and diffuse type without significant differences （P 〉 0.05）. After treatment with cytokine-induced killer （CIK） cells in the eighteen patients, the levels of CD25 and CD25 mRNA in the silence and inducement stages were all significantly increased with significant differences compared to those before treatment （P 〈 0.01）. [Conclusions] The low levels of CD25 and its mRNA expression are confirmed in hepatocellular carci- noma and have no association with pathological type of hepatocellular carcinoma. CD25 might be regarded as a valu-able indicator for observation of human cellular immune function and clinical efficacy. The expression of CD25 in peripheral blood of patients with hepatocellular carcinoma can be significantly up-regulated by CIK cells.