芹菜素对甲状腺癌BCPAP细胞生长及细胞周期的影响

Effects on cell viability and cell cycle of apigenin on BCPAP cell line of thyroid cancer

研究芹菜素对人乳头状甲状腺癌BCPAP细胞生长的抑制作用及对细胞周期的影响。采用MTT法检测不同浓度芹菜素在24h对BCPAP细胞的抑制作用,以及12.5,25.0,50.0μmol/L芹菜素分别在24,48,72h对BCPAP细胞的抑制作用;通过明场细胞形态学照片分析,对比不同浓度芹菜素对BCPAP细胞形态的影响,评价其对细胞生长的抑制作用。利用流式细胞仪检测BCPAP细胞周期和凋亡。结果表明,不同浓度（12.5~100.0μmol/L）芹菜素对BCPAP细胞的毒性有明显剂量和时间依赖性,其24h的IC50值为40.65μmol/L。芹菜素对BCPAP细胞的形态学变化有显著影响,高浓度芹菜素强烈抑制BCPAP细胞数目的增长。芹菜素可使BCPAP细胞周期的构成发生明显的变化并诱导细胞凋亡。芹菜素对BCPAP细胞有较明显的细胞毒性和生长抑制作用,其抑制机制可能是使BCPAP细胞生长停滞在G2/M期并诱导细胞凋亡,使得细胞生存率下降,从而抑制细胞活性和数目增长。

To investigate the effects on cell viability and cell cycle of apigenin on BCPAP cell line,the MTT assay was used to detect the growth inhibition rates of BCPAP cells at 24 hwhich cultured with apigenin in different concentrations,and of BCPAP cells at 24,48,72 hwhich cultured with 12.5,25,50μmol/L apigenin as well.The bright field images of cell morphology were analyzed.The effects were evaluated of different concentration of apigenin on the morphology of BCPAP cells,and the inhibition of cell growth.The cell cycle distribution was measured by flow cytometry.The different concentrations（12.5~100μmol/L）of apigenin had cytotoxicity on BCPAP cells,which were clearly dose-and time-dependent.The IC50 of 24h is 40.65μmol/L.There are significant effects of apigenin on the morphological changes of BCPAP cells,and the high concentration of apigenin strongly inhibited BCPAP cell growth in numbers.Cells were treated with apigenin which had significant effects on cell cycle of BCPAP cell,and induced apoptosis.Apigenin had significant cytotoxicity and inhibition of cell viability on thyroid cancer BCPAP cells.One of the mechanisms may be that it might make significant G2/M phase arrest and induced apoptosis,and a decrease of cell viability,then the cell number was declined.