菌质水溶性膳食纤维对小鼠肠蠕动与吸收的影响

Effects of fungal substance soluble dietary fiber on intestinal peristalsis and absorption in mice

研究菌质水溶性膳食纤维对小鼠肠蠕动与吸收的影响,为其在食品工业和医药保健领域应用提供理论依据。选取75只昆明种小鼠,随机分成5组:空白对照组、阳性对照组和3个受试组,分别灌胃生理盐水、50 mg/kg·BW的酚酞溶液、2.5g/kg·BW的水溶性膳食纤维、5g/kg·BW的水溶性膳食纤维、15g/kg·BW的水溶性膳食纤维。试验时间共13d,每天记录小鼠体重,小鼠小肠吸收试验和蠕动试验分别测定计算血清中木糖浓度、碳末推进率。结果表明:1菌质水溶性膳食纤维具有一定的抑制小鼠体重的作用(P<0.05),且抑制小鼠体重与菌质水溶性膳食纤维剂量呈正相关(P>0.05);2中剂量的菌质水溶性膳食纤维组小鼠小肠吸收功能的影响达显著水平(P<0.05);3菌质水溶性膳食纤维能促进小鼠小肠蠕动(高、中剂量达到极显著水平,P<0.01;低剂量达到显著水平,P<0.05),且小鼠小肠蠕动与菌质剂量呈正相关性(高剂量与低剂量的菌质水溶性膳食纤维组之间差异显著,P<0.05)。因此,一定量的菌质水溶性膳食纤维能抑制小鼠体重,具有促进小鼠小肠蠕动与吸收的作用。

In order to study on the effects of fungal substance soluble dietary fiber on the intestinal peristalsis and absorption in mice,75 kunming mice were randomly assigned to 5groups：blank control group fed with saline,control positive group fed with phenolphthalein solution of 50mg/kg·BW and three testing groups fed with low（2.5g/kg·BW）,middle（5g/kg·BW）and high（15kg/kg·BW）dosages of soluble dietary fiber in fungal substances.The results indicated that fungal substance soluble dietary fiber had some effects in inhibiting mice weight gain（P〈0.05）and there is a positive correlation between the dosages of soluble dietary fiber and the inhibition of weight gains,middle doses of soluble dietary fiber had a significant effect on intestinal peristalsis in mice（P〈0.05）,soluble dietary fiber enhanced intestinal peristalsis with great significance（P〈0.01）of high dosage and remarkable significance（P〈0.05）of middle dosage.The intestinal peristalsis is positively correlated with the dosage of the fungal substance soluble dietary fiber.The effect on intestinal peristalsis between high and low dosages of soluble dietary fiber is significantly different（P〈0.05）.