发酵肉制品中组胺含量测定方法的比较

Comparison of determination method for histamine content in fermented meat products

分别采用液相色谱法、中国国标法和中国国标改进法3种方法测定发酵肉制品中组胺含量,并对检测结果进行比较,旨在建立一种准确且适用于测定发酵肉制品中组胺含量的测定方法。结果表明,3种方法的标准曲线都呈现良好的线性关系;液相色谱法的精密度最高,相对标准偏差<1%,而中国国标法的精密度最差,相对标准偏差接近5%;液相色谱法和中国国标改进法加标回收率均高于90%以上,优于中国国标法,准确度满足试验要求。液相色谱法和中国国标改进法均能满足测定发酵肉制品中组胺含量的试验要求。

In order to build a suitable method for determination of histamine content in fermented meat products,liquid chromatography（HPLC）method,the national standard method and the improved national standard method were used to determine histamine content in fermented meat products.It was shown that the standard curve of the three methods showed a good linear relationship.HPLC method presented the highest precision with a good relative standard deviation（RSD〈1%）,while the national standard method exhibited the worst precision and the relative standard deviation was close to 5%.The range of recovery rate of both HPLC method and the improved national standard method were higher than 90% and were better than that of the national standard method.The results revealed that HPLC method and the improved national standard method could meet the determination of histamine content in fermented meat products experimental requirements.