油茶叶中黄酮的超声辅助提取及其抗氧化活性研究

Ultrasonic-assisted extracting and antioxidant activity of total flavonoids from leaves of camellia oleifera Abel

采用超声波辅助浸提油茶叶中的黄酮,优化其提取工艺,并研究其抗氧化活性。结果表明,优化后的提取工艺条件为:以50%乙醇溶液为提取剂,料液比1︰20(m︰V),提取温度70℃,超声功率300W下提取2次,每次30min。该条件下总黄酮得率为47.01 mg RE/g。油茶叶黄酮具有很强的DPPH·、ABTS+及·OH清除活性,IC50分别为15.12,21.15,410.00μg RE/mL。

Technology of ultrasonic-assisted method was optimized to extract flavonoids from leaves of camellia oleifera Abel,and antioxidant activity of flavonoids was investigated.It showed that the optimized conditions were 50% ethanol of extracting solvent,1∶20of material-liquid ratio（m ︰ V）,70 ℃ of extracting temperature,2times under 300 Wof electric power,30 min of ultrasonic treatment time,The optimized extraction yield of total flavonoids was 47.01 mg RE/g.The extraction of flavonoids from leaves of Camellia Oleifera Abel.showed strong free-radical scavenging activities of DPPH,ABTS and hydroxyl with 15.12,21.15 and 410μg RE/mL as IC50,respectively.