超临界CO_2萃取崖柏精油的研究

Research on supercritical CO_2 extraction of Thuja sutchuenensis essential oil

采用超临界CO2萃取法提取崖柏精油,通过正交试验对提取工艺进行优化,用GC—MS定性定量分析检测崖柏精油成分。结果表明,影响崖柏精油提取率的主要因素顺序为萃取压力、萃取温度、动态萃取时间;优化后的工艺条件为：物料装料系数0.68,萃取压力20 MPa,萃取温度50℃,动态萃取时间60min,静态萃取时间30 min,该条件下崖柏精油提取率为7.20%~7.31%。崖柏精油中共鉴定出28种化学成分,主要为萜烯类合化物,其中罗汉柏烯、α-雪松醇和花侧柏烯含量较高,其相对质量分数分别为45.50%,25.72%,7.11%。

Supercritical CO2 extraction method was applied to essential oil fromThuja sutchuenensis in the present study.The composition of the essential oil was identified by GC-MS.The extraction conditions were optimized by orthogonal tests.Results：The main factors influencing the extraction yield are extraction pressure,extraction temperature and extraction dynamic time.The Optimization of process：filling coefficient was 0.68,the extraction pressure and temperature were 20 MPa and 50 ℃,dynamic time was 60 min and static time was 30 min.The essential oil extraction rate were 7.20%~7.31% under this condition.28 components were identified in the Thuja sutchuenensis,primarily terpene compounds containing Thujopsene,α-cedrol and cuparene,accounting for 45.5%,25.41%and 7.11%,respectively.