超声波辅助提取野桂花蜜中多酚的工艺优化

Optimization of ultrasonic-assisted extraction of phenolic compounds from wild osmanthus-scented honey

为确定野桂花蜜中多酚化合物提取的最佳工艺参数,以野桂花蜜为主要原料,采用响应面优化野桂花蜜中多酚化合物的超声波辅助提取工艺。在单因素试验的基础上,以超声波时间、超声波温度、料液比、溶剂浓度为自变量,以多酚化合物得率为响应值,进行试验设计,研究自变量与响应值之间的关系。结果表明:优化得出的最佳工艺条件为溶剂浓度62%,料液比1︰10(m︰V),超声波时间21 min,超声波温度54℃。该工艺条件下多酚得率为68.27mg/10g。

The experiment is designed to get the optimum technological extraction conditions of phenolic compounds from wild osmanthus-scented honey.Response surface methodology was employed to optimize conditions for the ultrasonic-assisted extraction of phenolic compounds from wild osmanthus-scented honey,as main substrates.Based on the single factor experiments,the effect of solvent concentration,solid-liquid ratio,ultrasonic time and ultrasonic temperrature on phenolic rate were conducted as well as their relationship by experimental design.The optimal conditions for extracting phenolic from wild osmanthus-scented honey were determined as ethyl acetate volume fraction 62%,liquid to solid ratio 10mL/g,ultrasonic time of 20 min and ulrasonic temperature of 54 ℃.Under these conditions,the extraction efficiency of phenolic was 68.27mg/10 g.