摘要
目的 观察微小RNA(miRNA,miR)-187在前列腺癌中的表达及其对前列腺癌细胞增殖迁移的影响.方法 实时荧光定量聚合酶链反应(FQ-PCR)检测miR-187在6例高分化、18例中分化、9例低分化的前列腺癌及癌旁组织中的表达.将miR-187抑制物和阴性对照分别转染至前列腺癌细胞株DU145中.细胞计数法(CCK-8)、平板克隆法检测miR-187对DU145细胞增殖能力的影响;细胞划痕实验检测miR-187对DU145细胞株迁移能力的影响.结果 实时荧光定量聚合酶链反应检测结果提示,高分化组中癌组织和癌旁组织miR-187表达水平差异无统计学意义(P>0.05);中分化组和低分化组癌组织miR-187表达水平高于癌旁组织,分别为3.018、3.912倍,差异有统计学意义(P<0.05).中分化组癌组织中miR-187表达是高分化组癌组织的3.047倍(P<0.05);低分化组癌组织中miR-187表达是中分化组癌组织的1.787倍(P<0.05).CCK-8检测结果显示下调miR-187表达后,DU145细胞增殖速度较未处理组及miR-187 NC组下降58.65%和54.45%,差异有统计学意义(P<0.05).平板克隆形成实验结果显示下调miR-187表达后,细胞克隆形成率目较未处理组及miR-187 NC组降低14.5%,18.0%,差异有统计学意义(P<0.05).细胞划痕实验显示,培养24 h后miR-187 inhibitor组细胞间距离为未处理组和miR-187NC组的1.73、1.95倍.结论 miR-187在中、低分化的前列腺癌组织中高表达,抑制前列腺癌细胞DU145中的miR-187表达能够抑制前列腺癌增殖和迁移.
Objective To observe the microRNA (miRNA,miR)-187 expression in prostate cancer and its roles in prostate cancer cell proliferation and migration.Methods real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect miR-187 expression in six cases of well differentiated,eighteen cases of moderately differentiated and nine cases of poorly differentiated prostate cancer tissues.The miR-187 inhibitor and negative control were transfected into DU145 cells.Cell counting kit-8 (CCK-8) and colony formation assay were applied to measure cell proliferation;Cell wound healing assay was used to detect cell migration and invasion.Results The results of FQ-PCR suggest that:The expression level of miR-187 had no difference in well differentiated prostate cancer tissues and precancerous tissues.In moderately and poorly differentiated prostate cancers,the expression levels of miR-187 were higher than the precancerous tissues and the folds are 3.018 and 3.912 (P < 0.05).The expression of miR-187 was 3.047 fold higher in moderately differentiated prostate cancer than high and 1.787 fold higher in poorly differentiated prostate cancer than moderately.CCK-8 results showed that when miR-187 was inhibited,DU145 proliferation rate was declined to 58.65%,54.45% compared with the untreated group and miR-187 NC group (P <0.05).Colony formation experiments showed that after miR-187 was inhibited,the number of colony-forming cells were lower than the control group and the difference was statistically significant (P < 0.05).Cell wound healing assay showed the distance in miR-187 inhibitor was 1.73 and 1.95 folds than miR-187 NC and untreated group after 24 hours incubation.Conclusion The expression levels of miR-187 were higher in moderately and poorly differentiated prostate cancer tissues compared to precancerous tissues;Inhibition of miR-187 expression in DU145 prostate cancer cells can inhibit prostate cancer growth and migration.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第6期1285-1287,共3页
Chinese Journal of Experimental Surgery
基金
无锡市人民医院一研一题基金项目(YT201315)
关键词
前列腺癌
微小RNA
增殖
迁移
Prostate cancer
MicroRNA
Proliferation
Migration