摘要
目的 观察帕瑞昔布钠预先给药对大鼠肺缺血再灌注损伤时细胞凋亡的影响.方法 健康雄性SD大鼠72只,体质量250～ 300 g,随机分为3组（n=24）：假手术组（S组）、肺缺血再灌注组（I/R组）、肺缺血再灌注＋帕瑞昔布钠组（P组）.阻断右肺门60 min后再灌注120 min制备大鼠肺缺血再灌注模型,P组在夹闭右肺门前30 min腹腔注射帕瑞昔布钠5 mg/kg,S组和I/R组给予等体积生理盐水.于右肺门再灌注2h时处死大鼠,取肺组织.计算肺湿干重比和凋亡指数.免疫组织化学法检测肺组织B细胞淋巴瘤/白血病-2（bcl-2）、B细胞淋巴瘤/白血病-2相关X蛋白（bax）蛋白表达,计算bcl-2/bax比值,并观察病理学结果.结果 I/R组和P组肺组织湿干重比值分别为6.34 ±0.19和5.28 ±0.17、凋亡指数分别为（21.5±2.1）％和（11.6±0.8）％,bcl-2蛋白分别为0.29±0.07和0.45±0.09,bax蛋白分别为0.34 ±0.05和0.18 ±0.04,bcl-2/bax比分别为0.85±0.05和2.50±0.03,两组比较差异均有统计学意义（P＜0.05）.结论 帕瑞昔布钠预先给药可通过调节bcl-2和bax蛋白的表达抑制细胞凋亡,减轻肺缺血再灌注损伤.
Objective To investigate the effects of parecoxib on pneumocyet apoptosis during lung ischemia-reperfusion （I/R） injury in rats.Methods Seventy-two male SD rats were randomly divided into 3 groups （n =24 each）：sham operation group （S group）;I/R group and parecoxib group （P group）.The lung I/R was induced by occlusion of hilum of the right lung for 60 min followed by 120 min of reperfusion.In P group,parecoxib （5 mg/kg） was injected intravenously at 30 min before occlusion of hilum of the right lung.The rats were sacrificed at 2 h of reperfusion and then the lungs were removed for measurement of lung wet/dry weight ratio,apoptosis index,B cell lymphoma/leukemia-2 （bcl-2） and B cell lymphoma/leukemia-2 associated X protein （bax） protein expression,and microscopic examination.Bcl-2/bax ratio was calculated.Results Parecoxib preconditioning significantly attenuated the I/R-induced changes in lung wet/dry weight ratio （6.34 ±0.19 vs.5.28 ±0.17,P ＜0.05）,apoptosis index [（21.5 ±2.1）％ vs.（11.6 ±0.8）％,P＜0.05],bcl-2 protein expression （0.29 ±0.07 vs.0.45 ±0.09,P＜0.05） and bax protein expression （0.34 ±0.05 vs.0.18 ±0.04,P＜0.05）,and bcl-2/bax ratio （0.85 ± 0.05 vs.2.50 ± 0.03,P ＜ 0.05） in P group as compared with I/R group.Parecoxib preconditioning also ameliorated I/R-induced lung damage.Conclusion Parecoxib can inhibit pneumocyte apoptosis through regulating the expression of bcl-2 and bax protein,and ameliorate lung I/R injury.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第6期1368-1370,共3页
Chinese Journal of Experimental Surgery
基金
河南省医学科技攻关计划资助项目（201204026）
河南省基础研究计划资助项目（122300410075）
