摘要
目的 检测溃疡性结肠炎(UC)患者外周血中CC趋化因子配体20(CCL20)表达水平,分析CCL20表达水平与UC严重程度的关系.方法 入组52例UC患者(根据病情分为轻、中、重度)及30例健康体检者(对照组),分别收集外周血,Trizol法提取外周血中淋巴细胞总RNA,用实时定量聚合酶链反应(Real-time PCR)检测CCL20 mRNA表达水平;酶联免疫吸附试验(ELISA)检测外周血中CCL20蛋白质表达水平,独立样本t检验比较两组外周血中CCL20表达水平,方差分析比较轻、中、重度UC患者CCL20表达水平的差异.结果 UC组患者外周血中CCL20蛋白表达为(2 040.03 ± 730.07) ng/L,对照组外周血中CCL20蛋白表达为(550.01 ±70.71) ng/L,两组差异有统计学意义(P<0.05);轻、中、重度UC患者外周血中CCL20蛋白表达水平分别为(1 233.33±251.66)、(2300.01 ±300.00)、(3 034.56±152.75) ng/L,其差异有统计学意义(P<0.05).结论 UC患者外周血中CCL20表达增高,CCL20可能参与UC的发病.
Objective To explore the expression of chemokine (C-C motif) ligand 20 (CCL20) in peripheral blood of patients with ulcerative colitis (UC) and its relationship with severity of UC.Methods Fifty-two UC patients (18 mild cases,20 moderate cases,and 14 severe cases) and 30 healthy controls were enrolled.Peripheral blood of UC patients and healthy controls was collected.Trizol was used to extract total RNA in peripheral blood monocytes (PBMCs).Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect CCL20 mRNA expression level in PBMCs.CCL20 protein level in serum was assayed by enzyme-linked immunosorbent assay (ELISA).The difference in CCL20 expression level between two groups was compared by Student' s t test.The difference in CCL20 expression level among mild,moderate and severe UC patients was compared by analysis of variance (ANOVA).Results CCL20 protein expression level in serum of UC patients and healthy controls was (2 040.03 ± 730.07) and (550.01 ± 70.71) ng/L respectively (P < 0.05).CCL20 protein expression in serum of mild,moderate and severe UC patients was (1 233.33± 251.66),(2300.01± 300.00) and (3 034.56 ± 152.75) ng/L,respectively,with the difference being statistically significant (P < 0.05).Conclusion Serum CCL20 expression in UC patients is increased.CCL20 might be involved in the pathogenesis of UC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第6期1424-1425,共2页
Chinese Journal of Experimental Surgery