干化学法测定小鼠尾血电解质的可行性研究

Feasibility Study on Mouse Tail Blood Electrolytes Determination by Dry Chemical

本试验运用干化学法和湿化学法两种方法检测钙离子通道a1亚单位528位精氨酸突变为组氨酸(Cchl1a3-R528H)基因敲入小鼠的电解质水平,对两种不同检测方法进行比较,并分析其相关性。试验首先对该电解质分析仪的精密度进行检测,再选择同一小鼠,先取尾血使用干化学法检测电解质水平,再从眼球取全血,使用湿化学法检测电解质水平,两者进行比较。精密度试验结果显示干式电解质分析仪检测电解质Na+、Cl-、K+的变异系数分别为(2.06±0.18)%、(3.61±0.22)%、(4.40±0.19)%,均<4.5%。将两种方法所测得的电解质结果进行比较,结果显示差异无统计学意义(P>0.05);各项检测项目之间进行比较,结果显示呈线性相关(其中Na+、Cl-的r值均>0.975)。结果表明该电解质分析仪精密度高,稳定性好,且两种方法所检测电解质结果具有高度相关性及良好的可比性,后续试验可以使用干化学法替代湿化学法进行电解质检测。

In this experiment, dry chemical method and wet chemical method were used to detect electrolyte levels of Cchlla3-R528H gene knock-in mice,we compared the two different detection methods and analyzed their correlation. Firstly, we tested the precision of the electrolyte analyzer, and then selected the same mouse, collected the whole blood from tail with dry chemical method to detect electrolyte levels and collected the whole blood from the retro-orbital venous plexus with wet chemical method to detect electrolyte levels, and compared the two different detection meth- ods. The results showed that the CV of Na＋ ,CI and K＋ detected by dry electrolyte analyzer were （2.06 ±0.18）%, （3.61±0.22） % and （4.40± 0.19） %, respectively, all 〈 4.5%. Compared with the results detected by two different detection methods above-mentioned, the difference was not statistically significant （P〉0.05）, and there were linear positive correlations between the two detection methods in Na＋ ,CI- and K＋ （r of Na＋ and C1- were both 〉0. 975）. The result indica- ted that the dry electrolyte analyzer had high precision and good stability. The detected electro-lytes results by two detection methods were with high correlation and good comparability,subse- quent experiments could use dry chemical method to replace wet chemical method to detect elec- trolytes.