海南五指山猪Myf5与MyoD基因多态性分析

Polymorphism Analysis of Myf5 and MyoD Genes in Hainan Wuzhishan Pig

为研究海南五指山猪Myf5和MyoD基因的多态性分布情况,本试验通过PCR-RFLP方法对80头海南五指山猪进行了Myf5和MyoD基因型检测,并运用生物信息学方法对这两个基因的序列进行分析。结果发现,在Myf5基因第1外显子的HhaⅠ-RFLP位点上,五指山猪以CC和CG基因型为主;在Myf5基因第1外显子的Hsp92Ⅱ-RFLP位点上,五指山猪以AA基因型为主;在MyoD基因第1内含子的DdeⅠ-RFLP位点上,等位基因A在五指山猪中是优势等位基因。此外,Myf5和MyoD基因启动子区域和第1外显子处存在CpG岛。本研究得到海南五指山猪Myf5和MyoD基因的序列信息,为下一步五指山猪Myf5和MyoD基因的功能研究奠定基础。

This study was aimed to investigate the distribution of genetic polymorphisms of My f5 and MyoD genes in Hainan Wuzhishan pig. PCR-RFLP method was used to detect the My f5 and MyoD of 80 Wushishan pigs. Some characters of My f5 and MyoD genes were analyzed by bioin- formatics tools. The main results were as follows at the Hha I-RFLP locus of exon 1 in My f5 gene,CC and CG genetypes were major in Wuzhishan pig. At the Hsp92Ⅱ -RFLP locus of exon 1 in My f5 gene,AA genetype was major in Wuzhishan pig. At the Dde I -RFLP locus of intron 1 in MyoD gene,allele A was predominant in Wuzhishan pig. In addition, bioinformatics analysis re- vealed that My f5 and MyoD genes promoter and exon 1 region contained CpG islands. In this study,we obtained the sequences of My f5 and MyoD genes which laid a foundation for further studying their functions in Hainan Wuzhishan pig.