鸡源绿脓杆菌的分离鉴定及药敏试验

Isolation,Identification and Drug Susceptibility Test of Pseudomonas aeruginosain Chickens

为确诊引起秦皇岛某鸡场细菌性疾病的主要病原菌,分析其耐药情况,为临床用药和治疗提供依据,本试验对送检的病死鸡肝脏中分离得到的4株菌进行形态特征观察、生化鉴定、免疫荧光染色观察、细菌16SrRNA基因PCR扩增与测序分析及药敏试验。结果显示,4株分离菌均为革兰氏阴性、带绿色荧光的短杆菌,PCR扩增出的特异性条带与预期大小相吻合,确定为绿脓杆菌。药敏试验结果显示,分离株对庆大霉素、阿米卡星、大观霉素、头孢他啶、头孢曲松、左氧氟沙星、恩诺沙星、氟苯尼考、多黏菌素B和磷霉素高度敏感,对四环素和强力霉素中度敏感,而对新霉素、头孢噻肟、头孢噻吩、阿莫西林、氨苄西林、红霉素、复方新诺明和磺胺甲氧异唑耐药。提示,该分离菌对常用抗生素耐药情况严重,养殖场应根据药敏试验结果选择敏感药物进行合理用药。

In order to detect the main bacterial pathogens and analyze the drug resistance for pro- viding therapeutical guidance for drug administration, 4 stains were isolated from death chickens of a breeding chicken farm in Qinhuangdao area. All 4 isolates were identified by morphological characteristics,biochemical test,immunofluorescent staining, amplification of 16S rRNA genes by PCR, sequence analysis and drug susceptibility test. The results showed that all 4 isolates were gram-negative,bacillus brevis with green fluorescent,and the sequence of 16S rRNA gene of iso- lates were analyzed and test results showed that specific amplified band was consistent with the expected size. The isolated bacteria were identified to be Pseudornonas aeruginosa. Results of drug susceptibility test indicated that the bacterial isolates were highly sensitive to gentamycin,amika- cin, spectinomycin, ceftazidine, ceftriaxone, levofloxacin, enrofloxacin, florfenicol, polymyxin B and fosfomycin, intermidiate sensitive to tetracycline and doxycyclin, resistant to neomycin, cefo- taxime, cephalothin, amoxicillin, ampicillin, erythromycin, trimethoprim-sulfamethoxazole and ox- ygen sulfamethoxazole isoxazole. The results suggested that the resistances of Pseudomonas aeruginosa isolates to commonly used antibiotics were serious. So antibiotics should be reasonably selected based on the result of drug susceptibility test in this farm.