穿山龙TLC鉴别方法的改进研究

Study on the Improvement of TLC Identification of Dioscoreae nipponicae

目的：改进《中国药典》2010年版中穿山龙及其制剂骨龙胶囊中薯蓣皂苷元鉴别方法,为中国药典标准修订提高提供参考。方法：原薄层色谱法（TLC）鉴别方法采用三氯甲烷-甲醇（20∶0.2）为展开剂,以10%磷钼酸乙醇溶液为显色剂;改进后的方法以石油醚（60~90℃）-乙酸乙酯（4∶1）为展开剂,以10%硫酸乙醇溶液为显色剂。结果：原方法展开后斑点拖尾,磷钼酸显色后各成分斑点颜色差异小,斑点不够清晰。方法改进后,薯蓣皂苷元分离良好,重现性好,斑点清晰,其他成分无干扰;在骨龙胶囊穿山龙的鉴别应用中,效果良好。结论：该方法专属性强、重现性好,可以满足穿山龙和骨龙胶囊中薯蓣皂苷元的鉴别。

Objective： To improve the identification method of diosgenin in Dioscoreae nipponicae and its preparations in Chinese Pharmacopoeia （version 2010）, and to provide references for revision and perfection of the standards of Chinese Pharmacopoeia. Methods： The original TLC identification method used chloroform- methanol （20 ： 0.2） as the developing agent, and 10% phosphomolybdic acid with ethanol solution as the chromogenic agent. The improved method used petroleum ether （60-90 ℃）-ethyl acetate （4 ： 1） as the developing agent, and 10% sulfuric acid with ethanol solution as the chromogenic agent. Results： Using the original method, spots were unfolded trailed, the color difference of component spots was small and the spots were not clear enough after phosphomolybdic acid coloring. After improvement, diosgenin had good separation and repeatability, and clear spots without interference by other ingredients. Good results were achieved when this identification method was applied to Gulong capsules. Conclusion： This method has good specificity and repeatability and can be used for identification of diosgenin in Dioscoreae nipponicae and Gulong capsules.